Waseda University · 환경과학
Satoshi Tsuneda 교수의 연구실은 주로 환경 미생물학과 생물학적 처리 공정을 중심으로, 미생물 군집의 구조 및 기능, 특히 생물막 및 흙입자 형성에 기여하는 외부세포물질(EPS)의 역할을 연구하고 있습니다. 특히 흐름상태에서의 세포 부착, 질소 순환 미생물군집(질산화, 탈질소화)의 공간적 분포 및 기능적 상호작용, 장내 미생물군집과 장벽 기능의 관계 등 환경 및 의생명 공학 분야의 핵심 문제를 다룹니다. 복합적 분석 기법(16S rRNA 시퀀싱, NMR, 미세전기측정, 수학적 모델링 등)을 융합해 실질적인 생태계 기능을 규명하는 데 초점을 맞추고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
The influence of extracellular polymeric substances (EPS) on bacterial cell adhesion onto solid surfaces was investigated using 27 heterotrophic bacterial strains isolated from a wastewater treatment reactor. Cell adhesion onto glass beads was carried out by the packed-bed method and the results were discussed in terms of the amount of each EPS component produced and cell surface characteristics such as zeta potential and hydrophobicity. Protein and polysaccharides accounted for 75-89% of the EP
Epithelial barrier dysfunction has been implicated as one of the major contributors to the pathogenesis of inflammatory bowel disease. The increase in intestinal permeability allows the translocation of luminal antigens across the intestinal epithelium, leading to the exacerbation of colitis. Thus, therapies targeted at specifically restoring tight junction barrier function are thought to have great potential as an alternative or supplement to immunology-based therapies. In this study, we screen
The imbalance of gut microbiota is known to be associated with inflammatory bowel disease, but it remains unknown whether dysbiosis is a cause or consequence of chronic gut inflammation. In order to investigate the effects of gut inflammation on microbiota and metabolome, the sequential changes in gut microbiota and metabolites from the onset of colitis to the recovery in dextran sulfate sodium-induced colitic mice were characterized by using meta 16S rRNA sequencing and proton nuclear magnetic
This study evaluates the community structure in nitrifying granules (average diameter of 1600 mum) produced in an aerobic reactor fed with ammonia as the sole energy source by a multivalent approach combining molecular techniques, microelectrode measurements and mathematical modelling. Fluorescence in situ hybridization revealed that ammonia-oxidizing bacteria dominated within the first 200 mum below the granule surface, nitrite-oxidizing bacteria a deeper layer between 200 and 300 mum, while he
