The University of Tokyo · 생화학·유전·분자생물학
Shinichi Nishimura 교수의 연구실은 주로 해양 생물에서 유래한 생활물질(자연물)의 구조 규명과 생물학적 활성 메커니즘을 중심으로 연구를 진행하고 있습니다. 특히 항진균제 및 단백질 전이효소(GGTase I) 억제제로 작용하는 고산화된 알칼로이드, 폴리아세틸렌산 등 다양한 자연물의 분리 및 구조 결정에 중점을 두고 있으며, 세포막과의 상호작용, 스테롤 탐지 프로브 개발 등 생물막의 기능 이해에도 기여하고 있습니다. 최근에는 대사물질의 질량분석적 특징을 기반으로 새로운 스피로헤미아미날 유사 화합물의 발견과 합성에도 성공하여, 항균 활성과 관련된 신규 화합물 탐색에 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Massadine, a highly oxygenated alkaloid, was isolated from the marine sponge Stylissa aff. massa as an inhibitor of geranylgeranyltransferase type I (GGTase I). The structure of massadine has been deduced from spectral data. Massidine inhibited GGTase I from Candida albicans with an IC(50) value of 3.9 microM. [structure: see text]
Covering: up to 2019Nature furnishes bioactive compounds (natural products) with complex chemical structures, yet with simple, sophisticated molecular mechanisms. When natural products exhibit their activities in cells or bodies, they first have to bind or react with a target molecule in/on the cell. The cell membrane is a major target for bioactive compounds. Recently, our understanding of the molecular mechanism of interactions between natural products and membrane lipids progressed with the a
Two new polyacetylenic acids, corticatic acids D (2) and E (3), have been isolated from the marine sponge Petrosia corticata along with the known corticatic acid A (1) as geranylgeranyltransferase type I (GGTase I) inhibitors. Their structures were elucidated on the basis of spectroscopic and chemical methods. Compounds 1-3 inhibited GGTase I from Candida albicans with IC(50) values of 1.9-7.3 microM.
Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid membranes. However, only a limited number of chemical or protein probes have been developed so far for cytological analysis. Here we show that fluorescently-labeled derivatives of theonellamides act
Ferrichromes are a family of fungal siderophores with cyclic hexapeptide structures. Most fungi produce one or two ferrichrome-type siderophores. <i>Acremonium persicinum</i> MF-347833 produces ferrichrome-like potent Trojan horse antifungal antibiotics ASP2397 and AS2488053, the aluminum- and iron-chelating forms of AS2488059, respectively. Here, we show by gene sequencing followed by gene deletion experiments that <i>A. persicinum</i> MF-347833 possesses two nonribosomal peptide synthetase gen
Abstract A series of lipidic spirohemiaminals, designated streptoaminals, is reported. These were discovered by surveying the unique molecular signatures identified in the mass spectrometry data of the combined‐culture broth of Streptomyces nigrescens HEK616 and Tsukamurella pulmonis TP‐B0596. Mass spectrometry analysis showed that streptoaminals appeared as a cluster of ion peaks, which were separated by 14 mass unit intervals, implying the presence of alkyl chains of different lengths. The che
