Si Hyeock Lee
서울대학교 농업생명과학대학 생물학과 · 농업·생명과학
이 교수의 연구실은 곤충의 신경 생물학과 농약 내성 메커니즘을 중심으로 한 분자 생물학적 연구를 수행하고 있습니다. 특히 흡착효소(AChE)의 기능과 다양성, 농약에 대한 내성 유전자의 기전을 밝히는 데 초점을 맞추고 있으며, 꿀벌, 감자박테리아, 나무늘보기생선충 등 다양한 곤충 모델을 활용한 분석을 진행하고 있습니다. 유전자 발현 정량과 내성 기전의 분자 기초를 규명하는 데 있어 분자 생물학적 기법과 분석 기술을 융합한 연구가 특징입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
We investigated the molecular and kinetic properties of two acetylcholinesterases (AmAChE1 and AmAChE2) from the Western honey bee, Apis mellifera. Western blot analysis revealed that AmAChE2 has most of catalytic activity rather than AmAChE1, further suggesting that AmAChE2 is responsible for synaptic transmission in A. mellifera, in contrast to most other insects. AmAChE2 was predominately expressed in the ganglia and head containing the central nervous system (CNS), while AmAChE1 was abundant
Resistance to the rapid paralytic effects of pyrethroids in the permethrin-resistant (PE-R) strain of the Colorado potato beetle (CPB) is phenotypically similar to that of thekdrtrait of the house fly, which is caused by a single amino acid substitution (L1014F) in theVssc1voltage-sensitive sodium channel gene of that species. To test the hypothesis that thekdr-like trait of the PE-R strain of the CPB is caused by a similar mechanism, we amplified a 370-bp segment of the CPB ortholog of the hous
The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistochemistry revealed that BxACE-1 and BxACE-2 were distributed in neuronal tissues. In contrast, BxACE-3 was detected from some specific tissues and extracted without the aid of detergent, suggesting its
