Sun‐Mi Lee
고려대학교 환경생태공학부 · 공학
Sun-Mi Lee 교수의 연구실은 주로 생물학적 전환 기반의 지속 가능한 에너지 및 화학물질 생산을 목표로 하며, 라이노셀룰로오스 기반 바이오연료(예: 부탄올, 에탄올, 바이오디젤)의 효율적 생산을 위한 대사공학과 유전자 조작 기술을 핵심으로 합니다. 특히, 효모(Saccharomyces cerevisiae)와 유산균(Clostridium beijerinckii), 그리고 유산균형 유산균(Yarrowia lipolytica)을 활용한 대사 경로 최적화 및 효소 발현을 통해 기후 변화 대비 재생 가능 에너지 기술의 실현 가능성을 높이고자 합니다. 또한, 생물학적 억제제에 대한 내성 향상 및 대사 흐름 제어를 위한 단백질 기반 기술 개발도 함께 진행하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Butanol production by Clostridium beijerinckii NCIMB 8052 was investigated using both batch and continuous cultures containing suspended or immobilized cells. In the batch reactor, the initial addition of acetate and butyrate into the culture media was found not only to enhance solvent production but also to affect the ratio of acetone/butanol, which might result from the metabolic changes in solvent production. Furthermore, the addition of butyrate to the medium prevented strain degeneration du
The heterologous expression of a highly functional xylose isomerase pathway in Saccharomyces cerevisiae would have significant advantages for ethanol yield, since the pathway bypasses cofactor requirements found in the traditionally used oxidoreductase pathways. However, nearly all reported xylose isomerase-based pathways in S. cerevisiae suffer from poor ethanol productivity, low xylose consumption rates, and poor cell growth compared with an oxidoreductase pathway and, additionally, often requ
These results suggest that the xylose isomerase pathway should be the pathway of choice for efficient xylose fermentation in S. cerevisiae as it can outperform strains with the oxidoreductase pathway in terms of yield and ethanol production and xylose consumption rates. Consequently, the strain developed in this study could significantly improve the prospect of biofuels production from lignocellulosic biomass.
Abstract Lignocellulosic biomass has considerable potential for the production of fuels and chemicals as a promising alternative to conventional fossil fuels. However, the bioconversion of lignocellulosic biomass to desired products must be improved to reach economic viability. One of the main technical hurdles is the presence of inhibitors in biomass hydrolysates, which hampers the bioconversion efficiency by biorefinery microbial platforms such as Saccharomyces cerevisiae in terms of both prod
Abstract Lignocellulosic biomass shows high potential as a renewable feedstock for use in biodiesel production via microbial fermentation. Yarrowia lipolytica , an emerging oleaginous yeast, has been engineered to efficiently convert xylose, the second most abundant sugar in lignocellulosic biomass, into lipids for lignocellulosic biodiesel production. Yet, the lipid yield from xylose or lignocellulosic biomass remains far lower than that from glucose. Here we developed an efficient xylose‐utili
The membrane transporter ZitB responsible for Zn(II) efflux in Escherichia coli was studied by site-directed mutagenesis to elucidate the function of individual amino acid residues. Substitutions of several charged or polar residues, H53, H159, D163 and D186, located in predicted transmembrane domains resulted in loss of ZitB function. In contrast, neither the amino-terminal nor the carboxy-terminal regions, both histidine-rich, were required for function.
The petrochemical industry has grown to meet the need for massive production of energy and commodities along with an explosive population growth; however, serious side effects such as greenhouse gas emissions and global warming have negatively impacted the environment. Lignocellulosic biomass with myriad quantities on Earth is an attractive resource for the production of carbon-neutral fuels and chemicals through environmentally friendly processes of microbial fermentation. This review discusses
