Taichi Tenkumo 교수

This study aimed to fabricate a growth factor-releasing biodegradable scaffold for tissue regeneration. We prepared multishell calcium phosphate (CaP) nanoparticles functionalized with DNA, polyethyleneimine (PEI), protamine and octa-arginine (R8) and compared their respective transfection activity and cell viability measures using human mesenchymal stem cells. DNA-protamine complexes improved the transfection efficiency of CaP nanoparticles with the exception of those functionalized with R8. Th

The purpose of the present study was to confirm if proanthocyanidin-rich grape seed extract (GSE) had the ability to improve bone health such as bone loss, bone healing, and implant osseointegration (defined as the direct connection between bone tissue and an implant) in ovariectomized (OVX) animals. We demonstrated that daily oral administration of GSE prevented bone loss in the lumbar vertebrae and femur in OVX mice. In addition, osteoclastogenesis in the lumbar spine bone of OVX mice, as asse

Introducing integrated jaw models, rubric criteria and homework tasks to a total simulation training course may be a good approach for improving student performance in terms of dental diagnoses and treatment.

This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation. Streptococcus mutans and Aggregatibacter actinomycetemcomitans were used in suspension and as a biofilm on a titanium surface to test for the bactericidal effect. The bactericidal effect of the combination treatment was significantly higher than

The triple-functionalized CaP paste loading plasmid DNA encoding BMP-7 and VEGF and siRNA encoding TNF-α is a promising bioactive material for bone tissue repair.

本研究の目的は, 象牙質面にBMP-2処理する前の脱灰方法の違いが, 培養細胞のALP活性, および結合組織内での象牙質表面への硬組織形成と象牙質吸収に及ぼす影響を検討することである。実験1では, 象牙質片をEDTA (pH7.0), 塩酸テトラサイクリン (pH2.0), クエン酸 (pH1.0) で3分間脱灰後, 0, 5, 10μg/mlのrhBMP-2に10分間浸漬して, 歯根膜細胞を播種, 培養し, 1, 3, 5日後にALP活性を測定した。実験2では, 象牙質片をEDTA, 塩酸テトラサイクリン, クエン酸, PBSに3分間浸漬後, 0, 100, 400μg/mlのrhBMP-2に10分間浸漬し, ラット口蓋結合組織に移植して2, 4週後に象牙質片上に形成された硬組織の形成率と象牙質の吸収率を計測した。実験1の結果, BMP-2が10μg/mlの場合にEDTA群のALP活性が他の2群と比較して有意に高かった。実験2の結果, 硬組織形成率はBMP-2濃度が100μg/mlの場合にEDTA群が他の3群と比較して有意に高く, 象牙質吸収率はBMP-2が400μg/mlの場合にテ

Tnmd introduction treatment inhibits bone formation in artificial bone defect, however, the effect of that was dependent on non-viral gene transfection vector.

• The combination of silver ions and UV-A irradiation in bacterial suspensions generates hydroxyl, superoxide, and thiol radicals. • The yield of the generated DMPO-OH was increased by UV-A irradiation in silver ions and bacterial suspensions. • Silver ions in bacteria primarily function as promoters of UV-A photocatalysts. This study aimed to identify the ions used in disinfection treatments involving UV-A irradiation and to clarify the mechanism of free radical generation in three combinations

This report shows a simple and non-invasive method used to fabricate a fixating device for standardizing the three-dimensional positioning of focus, object, and dental film during intraoral radiographic examination in dogs for evaluation of hard tissue healing. To evaluate the validity of this device; the size of dental implants embedded in mandibular bone on two X-ray films taken either with or without device was compared. The difference in measurements expressed as a percentage while using the

With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2