Kyoto University · 농업·생명과학
Takayuki Miyazawa 교수의 연구실은 주로 동물 바이러스, 특히 고양이에 기생하는 레트로바이러스와 파보바이러스, 코로나바이러스 등의 분자 생물학적 특성과 병원성 기전을 중심으로 연구를 진행하고 있습니다. 특히 고양이면역결핍바이러스(FIV)의 수용체 기반 감염 메커니즘과 코로나바이러스의 유전자 다양성 및 역학을 규명하는 데 초점을 맞추고 있으며, 동물 모델을 통한 바이러스 감염 및 면역 반응 연구도 활발히 수행하고 있습니다. 또한 희귀 동물인 코알라의 고양이레트로바이러스(KoRV) 감염 상태를 분석함으로써 동물 질병의 분자 기전을 탐구하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Infrared Spectra of Polypeptides in Various Conformations: Amide I and II Bands1T. Miyazawa and E. R. BloutCite this: J. Am. Chem. Soc. 1961, 83, 3, 712–719Publication Date (Print):February 1, 1961Publication History Published online1 May 2002Published inissue 1 February 1961https://pubs.acs.org/doi/10.1021/ja01464a042https://doi.org/10.1021/ja01464a042research-articleACS PublicationsRequest reuse permissionsArticle Views2668Altmetric-Citations997L
Feline immunodeficiency virus (FIV) induces a disease similar to acquired immunodeficiency syndrome (AIDS) in cats, yet in contrast to human immunodeficiency virus (HIV), CD4 is not the viral receptor. We identified a primary receptor for FIV as CD134 (OX40), a T cell activation antigen and costimulatory molecule. CD134 expression promotes viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Infection is CXCR4-dependent, analogous to infectio
The Japanese isolate (TM1 strain) of feline immunodeficiency virus (FIV) which replicates in a feline CD4 (fCD4)-positive lymphoblastoid cell line (MYA-1 cells) was molecularly cloned from extrachromosomal closed circular DNA. The restriction map of the clone, termed pFTM 191 complete genome (CG), showed a considerable difference from that of the U.S. isolate (Petaluma strain) of FIV. The sequence homology in the long terminal repeat between the TM1 and Petaluma strain was 82%. The pFTM 191 CG w
Coronavirus disease of 2019 (COVID-19), which originated in China in 2019, shows mild cold and pneumonia symptoms that can occasionally worsen and result in deaths. SARS-CoV-2 was reported to be the causative agent of the disease and was identified as being similar to SARS-CoV, a causative agent of SARS in 2003. In this review, we described the phylogeny of SARS-CoV-2, covering various related studies, in particular, focusing on viruses obtained from horseshoe bats and pangolins that belong to <
Koala retrovirus (KoRV) is considered to be associated with leukemia, lymphoma and immunodeficiency-like diseases in koalas. We therefore conducted a pilot study of KoRV infection in five Queensland koalas in Kobe Municipal Oji Zoo. By polymerase chain reaction to detect partial env and pol genes of KoRV in genomic DNA isolated from whole blood and feces, all five koalas were found to be positive for KoRV proviruses. We succeeded in culturing koala lymphocytes from less than 1 ml blood for over
Feline parvovirus (FPV) was isolated rather frequently from the peripheral blood mononuclear cells (PBMCs) of cats in northern Vietnam by coculturing with MYA-1 cells (an interleukin-2-dependent feline T lymphoblastoid cell line) or Crandell feline kidney (CRFK) cells (a feline renal cell line). Efficiency of virus isolation was higher in MYA-1 cells than in CRFK cells. Interestingly, among the 17 cats from which FPV was isolated, 9 cats were positive for virus neutralizing (VN) antibody against
