Kyushu University · 농업·생명과학
Takeshi Zendo 교수의 연구실은 유산균에서 분리된 라이코코쿠스, 엔τ로바시움, 아필라크토박실러스 등 다양한 박테리오신 생산 미생물을 대상으로 신규 박테리오신의 도전적 발견과 기능 분석을 핵심으로 한다. 특히, nisin Q, lactococcin Q, kunkecin A 등 새로운 구조의 이중주형 또는 리더리스 박테리오신을 규명하고, 빠른 스크리닝 시스템을 통해 신속한 박테리오신 탐색에 기여한다. 연구는 식품 보존제 및 항균제로서의 응용 가능성을 고려한 안전성과 기능성에 초점을 맞추고 있다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the mature peptide and two in the leader sequence.
A new soybean isolate, Ent. mundtii QU 2 was found to be a class IIa bacteriocin producer. Factors influencing the bacteriocin production described herein are valuable for applications of the bacteriocins from Ent. mundtii strains.
A bacteriocin-producing strain, Lactococcus lactis QU 4, was isolated from corn. The bacteriocin, termed lactococcin Q, showed antibacterial activity only against L. lactis strains among a wide range of gram-positive indicator strains tested. Lactococcin Q was purified by acetone precipitation, cation exchange chromatography, and reverse-phase chromatography. Lactococcin Q consisted of two peptides, alpha and beta, whose molecular masses were determined to be 4,260.43 Da and 4,018.36 Da, respect
Bacteriocins produced by lactic acid bacteria (LAB) are expected to be safe antimicrobial agents. While the best studied LAB bacteriocin, nisin A, is widely utilized as a food preservative, various novel ones are required to control undesirable bacteria more effectively. To discover novel bacteriocins at the early step of the screening process, we developed a rapid screening system that evaluates bacteriocins produced by newly isolated LAB based on their antibacterial spectra and molecular masse
The developed system helps us to identify bacteriocins in the early stage of screening without any or with one-step pretreatment. This system is effective on not only detection of known bacteriocins but also identification of novel bacteriocins. Consequently, this system will accelerate discovery of novel bacteriocins.
<i>Apilactobacillus kunkeei</i> FF30-6 isolated from healthy honey bees synthesizes the bacteriocin, which exhibits antimicrobial activity against <i>Melissococcus plutonius</i>. The bacteriocin, kunkecin A, was purified through three-step chromatography, and mass spectrometry revealed that its relative molecular mass was 4218.3. Edman degradation of purified kunkecin A showed only the N-terminal residue, isoleucine. Hence, alkaline alkylation made the subsequent amino acid residues accessible t
