Xiaojun Peng 교수

Due to the biological importances of thiols, such as cysteine, homocysteine and glutathione, the development of optical probes for thiols has been an active research area in recent few years. This critical review focuses on the fluorescent or colorimetric sensors for thiols according to their unique mechanisms between sensors and thiols, including Michael addition, cyclization with aldehyde, cleavage of sulfonamide and sulfonate ester by thiols, cleavage of selenium-nitrogen bond by thiols, clea

Environment-related parameters, including viscosity, polarity, temperature, hypoxia, and pH, play pivotal roles in controlling the physical or chemical behaviors of local molecules. In particular, in a biological environment, such factors predominantly determine the biological properties of the local environment or reflect corresponding status alterations. Abnormal changes in these factors would cause cellular malfunction or become a hallmark of the occurrence of severe diseases. Therefore, in r

This tutorial review presents some recent developments in the construction and applications of cassettes based on resonance energy transfer between fluorescent dyes in the visible and infrared region. We focused on the contributions of different connections between the energy donor and acceptor according to the "through-space" and "through-bond" methods, and emphasised their applications in ratiometric sensing for the detection of ions and small molecules.

Mild chemical processes of various analytes and detection methods involving revolutionary strategies in the fields of analytical chemistry, biology and environmental sciences have been extensively developed. This critical review focuses on representative examples of mild chemical processes that can be used in fluorescent chemodosimeters for ion sensing (anions and cations). A systematisation according to the type of reaction mechanism is established. Numerous examples including extensions combin

Hypoxia, a quite universal feature in most solid tumors, has been considered as the "Achilles' heel" of traditional photodynamic therapy (PDT) and substantially impairs the overall therapeutic efficacy. Herein, we develop a near-infrared (NIR) light-triggered molecular superoxide radical (O₂^-•) generator (ENBS-B) to surmount this intractable issue, also reveal its detailed O₂^-• action mechanism underlying the antihypoxia effects, and confirm its applic

Traditional photothermal therapy requires high-intensity laser excitation for cancer treatments due to the low photothermal conversion efficiency (PCE) of photothermal agents (PTAs). PTAs with ultra-high PCEs can decrease the required excited light intensity, which allows safe and efficient therapy in deep tissues. In this work, a PTA is synthesized with high PCE of 88.3% based on a BODIPY scaffold, by introducing a CF₃ "barrier-free" rotor on the meso-position (tfm-BDP). In both the

A selective sensor 1 for the fluorescent imaging of Cd2+ in living cells has been designed and synthesized based on an internal charge transfer (ICT) mechanism. It can distinguish Cd2+ from Zn2+ and can be used in both general fluorescence intensity microscopy and ratiometric fluorescence microscopy.

New heptamethine cyanine dyes with an alkylamino group at the central position were found to exhibit a large Stokes shift (>140 nm) and strong fluorescence. They were suggested to be a new paradigm for excited-state intramolecular charge transfer (ICT). The configuration change of the bridgehead amine accompanying ICT was investigated in different viscosity and pH media.

Carbon dots (CDs), a kind of carbon material discovered accidentally, exhibit unexpected advantages in fluorescence imaging/sensing such as photostability, biocompatibility, and low toxicity. For emerging theranostics, an interdiscipline created by integrating therapy and diagnostics, CDs are good candidates when they are combined with targeted chemo/gene/photodynamic/photothermal therapeutic moieties. Here, the development of CDs in nanomedicine is reviewed from their use as original imaging ag

[reaction: see text] Phenyl-1H-anthra[1,2-d]imidazole-6,11-dione (1) and its derivatives (2 and 3) have been investigated as new colorimetric and ratiometric fluorescent chemosensors for fluoride. Acute spectral responses of 1 and 3 to fluoride in acetonitrile have been observed: an approximately 100 nm red shift in absorption and fluorescence emission and a very large ratiometric fluorescent response (Rmax/Rmin is 88 for sensor 1 and 548 for sensor 3). From the changes in the absorption, fluore

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is of great significance in the field of detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption and emission in the NIR region, tunable spectral properties, high photostability as well as a large Stokes shift. These properties are superior to those of conventional fluorogens, such as coumarin, fluorescein,

Transition metals (d-blocks) are recognized as playing critical roles in biology, and they most often act as cofactors in diverse enzymes; however, improper regulation of transition metal stores is also connected to serious disorders. Therefore, the monitoring and imaging of transition metals are significant for biological research as well as clinical diagnosis. In this article, efforts have been made to review the chemical sensors that have been developed for the detection of the first-row d-bl

Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules, and diffusion of reactive metabolites in live cells. Fluorescent molecular rotors are recently developed reagents used to determine the viscosity in solutions or biological fluid. Due to the complexity of live cells, it is important to carry out the viscosity determinations in multimode for high reliability and accuracy. The first molecular rotor (RY3) capable of dual mode f

Polydiacetylenes (PDAs), a family of conjugated polymers, have very unique electrical and optical properties. Upon environmental stimulation, such as by viruses, proteins, DNAs, metal ions, organic molecules etc., the blue PDAs can undergo a colorimetric transition from blue to red, which is accompanied by a fluorescence enhancement. Since the first report on polymerized diacetylene molecules as sensors of influenza virus, the development of efficient sensory systems based on PDAs continues to b

Tumor hypoxia has proven to be the major bottleneck of photodynamic therapy (PDT) to clinical transformation. Different from traditional O₂ delivery approaches, here we describe an innovative binary photodynamic O₂-economizer (PDOE) tactic to reverse hypoxia-driven resistance by designing a superoxide radical (O₂^•-) generator targeting mitochondria respiration, termed SORgenTAM. This PDOE system is able to block intracellular O₂ consumption