Yeon Ho Je
서울대학교 농업생명과학과 · 생화학·유전·분자생물학
Yeon Ho Je 교수의 연구실은 바이러스 기반 단백질 발현 시스템과 곤충 병원성 바이러스의 기생 메커니즘을 중심으로 연구를 진행하고 있습니다. 특히 바 큐로바이러스가 곤충 세포 내에서 단백질을 효율적으로 발현하고, 오염체 형성 과정을 이용해 외래 단백질을 안정적으로 함양하는 기술 개발에 초점을 맞추고 있으며, 이는 단백질 공학 및 생물학적 살충제 개발에 응용됩니다. 또한 곤충 호르몬 조절 물질을 탐색하고, 기생 곤충 병원성 진균에서 유래한 새로운 곤충 성장 조절 물질을 발굴하는 데에도 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Current baculovirus expression systems typically produce soluble proteins that accumulate within the infected insect cell or are secreted into the growth medium. A system has now been developed for the incorporation of foreign proteins, along with the matrix protein, polyhedrin, into baculovirus occlusion bodies. Initial studies showed that a recombinant virus expressing a translational fusion between polyhedrin and GFP did not form occlusion bodies. However, a baculovirus coexpressing native po
Using RNA-seq, we have identified groups of immune-regulated and juvenile hormone-regulated genes that are suppressed by infection with active AcMNPV. This information and further studies on the regulation of host gene expression by AcMNPV will provide the tools needed to enhance the utility of the virus as an effective protein expression system and as an insecticide.
Abstract Insect growth regulators (IGRs) are effective alternatives to chemical insecticides because of their specificity and low environmental toxicity. Entomopathogenic fungi are an important natural pathogen of insects and have been developed as biological control agents. They produce a wide range of secondary metabolites such as antibiotics, pesticides, growth‐promoting or inhibiting compounds and insect attracting agents. In this study, to explore novel IGR substances from entomopathogenic
In this study, we successfully silenced SBV in its host, A. cerana, by the application of exogenous dsRNA produced from an entomopathogenic bacteria, Bt. These results suggested that Bt could be a useful dsRNA production platform to control viral pathogens in their host insects. © 2019 Society of Chemical Industry.
Insect-killing (entomopathogenic) fungi have high potential for controlling agriculturally harmful pests. However, their pathogenicity is slow, and this is one reason for their poor acceptance as a fungal insecticide. The expression of bumblebee, Bombus ignitus, venom serine protease (VSP) by Beauveria bassiana (ERL1170) induced melanization of yellow spotted longicorn beetles (Psacothea hilaris) as an over-reactive immune response, and caused substantially earlier mortality in beet armyworm (Sp
