The University of Osaka · 생화학·유전·분자생물학
요시에 하라다 교수의 연구실은 나노스케일의 열전달 메커니즘과 생체 내 열전도도를 정밀하게 측정하는 데 초점을 맞추고 있습니다. 특히 나노히터/나노온도계를 활용한 세포 내 열전도도 측정 기술을 개발하여, 암세포(HeLa, MCF-7)의 열적 특성에 대한 새로운 이해를 제공하고 있습니다. 또한, 액틴-미오신 상호작용의 단일 분자 수준에서의 동역학을 실시간 영상으로 관찰하는 생물물리학적 연구도 수행하고 있습니다. 이와 더불어, GC-rich DNA 서열을 고선택적으로 인식하는 페닐아미드 분자 설계 및 기질 특성 분석을 통해 유전자 조절 분야의 응용 가능성도 탐색하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Understanding heat dissipation processes at nanoscale during cellular thermogenesis is essential to clarify the relationships between the heat and biological processes in cells and organisms. A key parameter determining the heat flux inside a cell is the local thermal conductivity, a factor poorly investigated both experimentally and theoretically. Here, using a nanoheater/nanothermometer hybrid made of a polydopamine encapsulating a fluorescent nanodiamond, we measured the intracellular thermal
We used video-fluorescence microscopy to directly observe the sliding movement of single fluorescently labeled actin filaments along myosin fixed on a glass surface. Single actin filaments labeled with phalloidin-tetramethyl-rhodamine, which stabilizes the filament structure of actin, could be seen very clearly and continuously for at least 60 min in 02-free solution, and the sensitivity was high enough to see very short actin filaments less than 40 nm long that contained less than eight dye mol
N-Methylpyrrole (Py)-N-methylimidazole (Im) polyamides are small organic molecules that can recognize predetermined DNA sequences with high sequence specificity. As many eukaryotic promoter regions contain highly GC-rich sequences, it is valuable to synthesize and characterize Py-Im polyamides that recognize GC-rich motifs. In this study, we synthesized four hairpin Py-Im polyamides 1-4, which recognize 5'-GCGC-3' and investigated their binding behavior with surface plasmon resonance assay. Py-I
