Kyushu University · 생화학·유전·분자생물학
요시키 카타야마 교수의 연구실은 생체 신호 분자 인식과 반응성 고분자를 중심으로 한 신소재 개발에 주력하고 있습니다. 단백질 키나제(A, C, caspase-3 등)의 활성화를 감지하는 스위치 기능을 가진 고분자-펩티드 접합체를 설계하여, 생체 내 신호 전달 경로를 실시간으로 탐지하고 제어하는 기술을 개발하고 있습니다. 특히, 온도나 인산화 반응에 의해 구조가 변화하는 스마트 고분자와 형광 프로브를 활용한 생체 분자 감지 시스템을 선도하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
New polymer−peptide conjugates, NIPAM−PEP and NIPAM−PEPEP, were designed and synthesized. These graft-type polymers contained a substrate peptide of protein kinase A (PKA), which forms one of the most important intracellular signals in cellular signal transduction. The NIPAM−PEP containing the N-isopropylacrylamide unit and the substrate peptide unit raised its lower critical solution temperature (LCST) from 36.7 to 40 °C in response to phosphorylation by activated PKA. The NIPAM−PEPEP containin
A fluorescent probe using a novel 'spin exchange' concept was developed for monitoring nitric oxide (NO) production. The probe is composed of 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) labeled with acridine and N-dithiocarboxysarcosine (DTCS)-Fe(II) complex. When the non-fluorescent acridine-TEMPO was incubated with DTCS-Fe(II) complex in buffer solution, the nitroxide radical in the acridine-TEMPO interacted with the Fe(II) through a redox interaction. This interaction recovered the fluoresce
We describe two types of artificial gene-regulation systems responding to cyclic AMP-dependent protein kinase (PKA) or caspase-3. These molecular systems use newly synthesized cationic polymers, PAK and PAC. The PAK polymer includes substrate oligopeptide for PKA, ARRASLG, as receptor of PKA signal, while the PAC polymer possesses oligopeptide that is comprised of a substrate sequence of caspase-3, DEVD, and a cationic oligolysine, KKKKKK. These polymers formed stable complexes with DNA to total
The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences was designed using Scansite and screened by MALDI-TOF MS and kinase activity assays for PKC isozyme-specificity. A peptide (Alphatomega; H-FKKQGSFAKKK-NH(2)) with high specificity for PKC alpha relative to other isozymes was identified. The peptide was phosphorylated to a greater extent by tissue lysates from B16 melanoma, HepG2, and human breast cancer, which had h
