Kyoto University · 생화학·유전·분자생물학
요시다 요시노리 교수의 연구실은 인간 induced pluripotent stem cells(iPSC)를 활용한 심장 질환 모델링과 약물 스크리닝을 핵심으로 하며, 특히 칼모듈린 유전자 변이가 관련된 조루성 린드롬 증후군(LQTS) 및 콜레스테롤성 심실세동(CPVT)의 분자 기전을 iPS 세포 유래 심근세포를 통해 규명하고 있습니다. 또한, 가상현실 기반의 허브틱 치과 교육 시스템 개발을 통해 의료 교육 분야의 혁신을 도모하고 있으며, 고내성 고유리 전이 온도 고분자 합성 및 응용 연구도 병행하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Induced pluripotent stem cells (iPSCs) are reprogrammed cells that have features similar to embryonic stem cells, such as the capacity of self-renewal and differentiation into many types of cells, including cardiac myocytes. Although initially the reprogramming efficiency was low, several improvements in reprogramming methods have achieved robust and efficient generation of iPSCs without genomic insertion of transgenes. iPSCs display clonal variations in epigenetic and genomic profiles and cellu
Calmodulin is a ubiquitous Ca2+ sensor molecule encoded by three distinct calmodulin genes, CALM1-3. Recently, mutations in CALM1-3 have been reported to be associated with severe early-onset long-QT syndrome (LQTS). However, the underlying mechanism through which heterozygous calmodulin mutations lead to severe LQTS remains unknown, particularly in human cardiomyocytes. We aimed to establish an LQTS disease model associated with a CALM2 mutation (LQT15) using human induced pluripotent stem cell
We recapitulated the electrophysiological features of CPVT-derived hiPSC-CMs using electrical pacing. The development of DADs in the presence of isoproterenol was significantly suppressed by S107. Our model provides a promising platform to study disease mechanisms and screen drugs.
A virtual reality (VR) haptic dental training system could be a promising tool for future dental education. One major challenge is to develop a virtual tooth model which similarly reflected a real tooth having multiple layers with different mechanical hardness in each layer. The multi-layered virtual tooth model was successfully constructed in our virtual system. The constructed model allows us to feel tooth cutting which is similar to that with a real tooth. Through a cutting experiment by usin
The myelodysplastic syndromes are a heterogeneous group of clonal hematopoietic disorders predominantly affecting the elderly. Patients frequently develop acute leukemia, but the majority suffer from the consequences of bone marrow failure. The underlying acquired genetic abnormality is the inadequate production of dysplastic and poorly functional cells resulting from defective differentiation and premature cell death of the abnormal hematopoietic clone. Although the pathogenesis is unknown, rec
Ester or cyano substituted tetracyclo [4.4.0.12,5.17,10]dodec-3-enes (1) were synthesized and their metathesis ring-opening polymerization was examined. The tungsten-based ternary catalyst system polymerized them very well. The polymers showed high glass transition temperatures (Tg) and no evidence of crystallization (e.g., the Tg of the polymer derived from 8-methyl-8-methoxycarbonyl substituted monomer (1a) was 207°C, and colorless transparent films could be casted from the solution of the pol
Induced pluripotent stem cells (iPSCs) from type 1 long QT (LQT1) patients can differentiate into cardiomyocytes (CMs) including ventricular cells to recapitulate the disease phenotype. Although optical recordings using membrane potential dyes to monitor action potentials (APs) were reported, no study has investigated the disease phenotypes of cardiac channelopathy in association with the cardiac subtype at the single-cell level. We induced iPSC-CMs from three control and three LQT1 patients. Si
