Kyoto University · 생화학·유전·분자생물학
Yuki Takahashi 교수의 연구실은 소형외소세포(소외소포, sEVs)의 생체 내 동역학과 기능을 중심으로, 특히 혈액 내 sEV의 분비 및 청소 메커니즘, 표면 인지질(예: 인지질세라마이드)이 면역세포 인식에 미치는 영향을 규명하고자 합니다. 또한, 암세포 유래 외소포의 종양 내 분포와 종양 성장에 미치는 영향, 그리고 외소포를 약물 전달재로 활용하기 위한 내부 조작 기술 개발도 진행 중입니다. 연구는 주로 생체 내 추적, 유전자 조작, 생물광학적 분석을 융합한 정량적 접근을 통해 전개됩니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Small extracellular vesicles (sEVs) are important mediators of cell-cell communication with respect to diverse physiological processes. To further understand their physiological roles, understanding blood sEV homoeostasis in a quantitative manner is desired. In this study, we propose novel kinetic approaches to estimate the secretion and clearance of mouse plasma-derived sEVs (MP-sEVs) based on the hypothesis that blood sEV concentrations are determined by a balance between the secretion and cle
Exosomes are extracellular vesicles released by various cell types and play roles in cell-cell communication. Several studies indicate that cancer cell-derived exosomes play important pathophysiological roles in tumor progression. Biodistribution of cancer cell-derived exosomes in tumor tissue is an important factor for determining their role in tumor proliferation; however, limited studies have assessed the biodistribution of exosomes in tumor tissues. In the present study, we examined the effe
Extracellular vesicles (EVs) are small membrane vesicles secreted from cells and have great potential as drug delivery carriers. Surface proteins on EV membranes might play roles in pharmacokinetics. One method which can be used to study the role of surface membrane of EV is to modify the inner space of EV. In the present study, we constructed a plasmid DNA expressing a fusion protein of Gag protein derived from Moloney murine leukemia virus (Gag) and Gaussia luciferase (gLuc) (Gag-gLuc) to modi
Small extracellular vesicles (sEVs) are important mediators of intercellular communication with respect to diverse pathophysiological processes. Here, we determined novel phosphatidylserine (PS)-deficient sEV subpopulations as a major somatic cell-derived sEV subpopulation in blood because of long blood circulation half-life through escape from macrophage uptake. PS<sup>(-)</sup>-sEVs were identified in various cultured cells as a minor population. However, as a result of rapid uptake of PS<sup>
