서울대학교 · Engineering
유경경 교수의 연구실은 주로 마이코박터이움 및 샤론엘라와 같은 내세포성 병원체의 매크로파지 내 생존 메커니즘을 밝히는 데 초점을 맞추고 있습니다. 특히 병원체가 융합된 포식포의 pH 조절과 리소좀과의 융합 억제를 통해 내성과 병원성을 확보하는 과정을 분자생물학적·세포생물학적 방법으로 규명하고 있습니다. 또한, 이와 관련해 약물 전달 시스템, 특히 리포좀 기반의 나노입자를 활용한 항생제 및 기능성 화장료의 효율적 세포 내 전달 전략을 개발하고 있습니다. 이는 감염 치료 및 미용 화장품의 혁신적 제형 개발에 기여하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
The virulence of Salmonella typhimurium for mice results, in part, from its ability to survive after phagocytosis by macrophages. Although it is generally agreed that intracellular bacteria persist in membrane-bound phagosomes, there remains some question as to whether these phagosomes fuse with macrophage lysosomes. This report describes the maturation of phagosomes containing S. typhimurium inside mouse bone marrow-derived macrophages. Macrophages were infected briefly and incubated for variou
Phagocytosis in macrophages is often studied using inert polymer microspheres. An implicit assumption in these studies is that such particles contain little or no specific information in their structure that affects their intracellular fate. We tested that assumption by examining macrophage phagosomes containing different kinds of particles and found that although all particles progressed directly to lysosomes, their subsequent fates varied. Within 15 min of phagocytosis, >90% of phagosomes cont
Mycobacterium avium is an intracellular pathogen that can invade and multiply within macrophages of the reticuloendothelial system. Current therapy is not highly effective. Particulate drug carriers that are targeted to the reticuloendothelial system may provide a means to deliver antibiotics more efficiently to M. avium-infected cells. We investigated the formulation of the antibiotics ciprofloxacin and azithromycin in liposomes and tested their antibacterial activities in vitro against M. aviu
Mycobacterium avium is a facultative intracellular pathogen that can survive and replicate within macrophages. We tested the hypotheses that survival mechanisms may include alteration of phagosomal pH or inhibition of phagosome-lysosome fusion. M. avium was surface labeled with N-hydroxysuccinimidyl esters of carboxyfluorescein (CF) and rhodamine (Rho) to enable measurement of the pH of individual M. avium-containing phagosomes and the interactions of bacterium-containing phagosomes with labeled
To develop a more effective transdermal delivery method for lipophilic functional cosmetic compounds such as retinol, we formulated various deformable liposomes and compared their transdermal delivery efficiency with those of neutral or negatively-charged conventional liposomes. We tested the deformability of liposomes containing edge activators such as bile salts, polyoxyethylene esters and polyoxyethylene ethers. As indicators of deformability, we used the passed volume and phospholipid ratios
