Yusuke Sato 교수

DNA has the potential to achieve a controllable macromolecular structure, such as hydrogels or droplets formed through liquid-liquid phase separation (LLPS), as the design of its base sequence can result in programmable interactions. Here, we constructed "DNA droplets" via LLPS of sequence-designed DNA nanostructures and controlled their dynamic functions by designing their sequences. Specifically, we were able to adjust the temperature required for the formation of DNA droplets by designing the

Rapid progress in nanoscale bioengineering has allowed for the design of biomolecular devices that act as sensors, actuators, and even logic circuits. Realization of micrometer-sized robots assembled from these components is one of the ultimate goals of bioinspired robotics. We constructed an amoeba-like molecular robot that can express continuous shape change in response to specific signal molecules. The robot is composed of a body, an actuator, and an actuator-controlling device (clutch). The

Photovoltaic (PV) systems having rechargeable batteries are prone to be complex and costly because multiple converters are necessary to individually regulate a load, PV panel, and battery. This paper proposes novel nonisolated multiport converters (MPCs) integrating a bidirectional pulsewidth modulation (PWM) converter and phase-shift-switched capacitor converter (PS-SCC) for standalone PV systems. A PWM converter and PS-SCC are integrated by reducing the total switch count, realizing the simpli

Phase separation is a key phenomenon in artificial cell construction. Recent studies have shown that the liquid-liquid phase separation of designed-DNA nanostructures induces the formation of liquid-like condensates that eventually become hydrogels by lowering the solution temperature. As a compartmental capsule is an essential artificial cell structure, many studies have focused on the lateral phase separation of artificial lipid vesicles. However, controlling phase separation using a molecular

Abstract Self‐assembly of designed nanostructures on a lipid membrane offers a powerful approach for enhancing the functionality of its surface. Here, the environment‐dependent assembly of blunt‐ended DNA origami structures on a phase‐separated lipid‐bilayer membrane consisting of liquid‐disordered (Ld) and solid‐ordered (So) phases is reported. Fluorescence microscopy and atomic force microscopy (AFM) imaging reveal that DNA origami structures preferentially bind to the So phase. At higher conc

Liquid-liquid phase separation (LLPS) of biopolymer molecules generates liquid-like droplets. Physical properties such as viscosity and surface tension play important roles in the functions of these droplets. DNA-nanostructure-based LLPS systems provide useful model tools to investigate the influence of molecular design on the physical properties of the droplets, which has so far remained unclear. Herein, we report changes in the physical properties of DNA droplets by sticky end (SE) design in D

The creation of artificial cells is an immensely challenging task in science. Artificial cells contribute to revealing the mechanisms of biological systems and deepening our understanding of them. The progress of versatile biological research fields has clarified many biological phenomena, and various artificial cell models have been proposed in these fields. Microfluidics provides useful technologies for the study of artificial cells because it allows the fabrication of cell-like compartments,

An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

Deoxyribonucleic acid (DNA) nanotechnology, a frontier in biomedical engineering, is an emerging field that has enabled the engineering of molecular-scale DNA materials with applications in biomedicine such as bioimaging, biodetection, and drug delivery over the past decades. The programmability of DNA nanostructures allows the precise engineering of DNA nanocarriers with controllable shapes, sizes, surface chemistries, and functions to deliver therapeutic and functional payloads to target cells

Rapid-single-flux-quantum digital-to-analogue converters (RSFQ-DACs) are now under development for ac voltage standard applications. The voltage multiplier (VM), which precisely amplifies the input voltage, is a key component for RSFQ-DACs. Because the amount of bias current for a conventional VM increases in proportion to its multiplication factor, we have been looking for a VM device which is operated on a different principle. In this paper, we report our design of a 1000-fold VM comprising do

Multi-power-source systems containing multiple power sources have gained considerable attention since the advent of renewable energy sources, electric vehicles, etc. These systems tend to increase complexity and costs because multiple converters in proportion to the number of power sources are required to individually control each source. Multi-port converters (MPCs) that combine multiple converters into a single unit have been reported to achieve simplified systems at lower costs. Most conventi

Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve to concentrate specific molecules, thus enhancing biochemical reactions. Artificial LLPS systems have been utilized to construct synthetic cell models, employing a range of synthetic molecules. LLPS systems based on DNA nanotechnology are particularly notable for their designable chara

The formation of biomolecular condensates via phase separation relates to various cellular functions. Reconstituting these condensates with designed molecules facilitates the exploration of their mechanisms and potential applications. Sequence-designed DNA nanostructures enable the investigation of how structural design influences condensate formation and the construction of functional artificial condensates. Despite the high designability of DNA-based condensates, free nanostructures that do no

Constrained clustering is attracting attention as a useful way for grouping a data set into intended clusters using users' feedback. We develop an interactive document clustering method by employing constrained clustering in order to group patent documents into some technological categories based on their contents. This method aims to progressively improve the accuracy of clustering to repeat both the assigning of appropriate cluster to documents and applying constrained clustering. We evaluate