Seong Ho Kang
경희대학교 응용화학과 · 생화학·유전·분자생물학
성호 강 교수의 연구실은 생체 분자의 표면 상호작용, 단일 분자 동역학, 그리고 나노구조를 활용한 생물 센서 기술을 중심으로 연구를 진행하고 있습니다. 특히, 단일 분자 수준에서 DNA 및 단백질의 광학적 동역학을 실시간으로 관찰하는 캐피라리 전기영동, 총내반사 형광현미경, 메탈증강형 형광 기반 센서 기술을 응용하여 생물학적 분석의 민감도와 해상도를 극대화하고 있습니다. 또한, 생체 적합성 있는 광교환성 분자와 나노소재를 활용한 실시간 세포 영상 기술 개발에도 주력하고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
The conformational dynamics and adsorption/desorption behavior of individual lambda-DNA molecules at liquid-solid interfaces were monitored by imaging within the evanescent field layer using total internal reflection fluorescence microscopy. At a fused-silica surface, molecular conformation and adsorption behavior were found to depend on both pH and buffer composition. A histogram of individual lambda-DNA adsorption durations measured by hydrodynamically flowing molecules along the interface exh
A new diarylethene-derived photochromic compound, with little cytotoxicity, plasma membrane-permeability, and efficient photoswitchability in cells upon alternative UV and visible light irradiation, was synthesized and applied to live cell imaging.
The behavior of individual molecules of R-phycoerythrin (RPE) was monitored by fluorescence imaging at various pHs and ionic strengths within the evanescent-field layer (EFL) at a water/fused-silica interface. Above the isoelectric point (pI), the individual protein molecules moved between exposures with random motion. As the pH approached the pI of the protein, the RPE molecules were partially adsorbed onto the fused-silica surface. The residence time and the number of molecules within the EFL
A novel multimodal method for peptide mapping of proteins by multiplexed capillary electrophoresis (CE) is presented. By combining charge to size separations in four different channels and micellar electrokinetic chromatography for hydrophobicity-based separations in two different channels in a 96-capillary array, peptide fragments of digested proteins were readily resolved and showed unique fingerprints. Each capillary spanned several diodes in a photodiode array (PDA) for absorption measuremen
Abstract Microchip‐based capillary electrophoresis (ME) raises fascinating possibilities for new analytical technologies particularly in the detection of DNA fragments in modern biology. The ME technique has gained considerable popularity with significant progress being made in the development of miniaturized microfluidic devices in micro‐volume analysis methods. The ME offers the potential for highly efficient, simultaneous analysis of a large number of biologically important molecules in genom
