The University of Osaka · 의학
이 교수의 연구실은 주로 말라리아 parasite의 유전자 조절 메커니즘과 병원체-숙주 상호작용을 중심으로 한 기초생물학 연구를 수행하고 있습니다. 특히 간기 단계의 말라리아 parasite에서 작용하는 전사인자(AP2-L)와 인공염색체를 이용한 유전자 기반 기술 개발에 초점을 맞추고 있으며, 해충 및 체액 면역 반응에 관여하는 단백질의 기능 규명도 함께 진행하고 있습니다. 이는 새로운 약물 및 백신 타겟을 발굴하는 데 기여합니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Invertebrate animals, which lack adaptive immune systems, have developed defense systems, so-called innate immunity, that respond to common antigens on the surface of potential pathogens. One such defense system is involved in the cellular responses of horseshoe crab hemocytes to invaders. Hemocytes contain two types, large (L) and small (S), of secretory granules, and the contents of these granules are released in response to invading microbes via exocytosis. Recent biochemical and immunologica
Two N-terminal fragments of α(A)-chain and β(B)-chain in human fibrinogen have been isolated from a plasmic hydrolyzate. The fragment from the α(A)-chain consisted of 43 amino acid residues including two half-cystine residues. On treatment with thrombin, this fragment produced two other peptides in addition to fibrinopeptide A and its analogues. One was a tripeptide, Gly-Pro-Arg, and the other a peptide containing 24 amino acid residues having N-terminal valine. The partial amino acid sequence o
Liver-stage malaria parasites are a promising target for drugs and vaccines against malaria infection. However, little is currently known about gene regulation in this stage. In this study, we used the rodent malaria parasite Plasmodium berghei and showed that an AP2-family transcription factor, designated AP2-L, plays a critical role in the liver-stage development of the parasite. AP2-L-depleted parasites proliferated normally in blood and in mosquitoes. However, the ability of these parasites
Novel antithrombin molecules were identified from the ixodidae tick, Haemaphysalis longicornis. These molecules, named madanin 1 and 2, are 7-kDa proteins and show no significant similarities to any previously identified proteins. Assays using human plasma showed that madanin 1 and 2 dose-dependently prolonged both activated partial thromboplastin time and prothrombin time, indicating that they inhibit both the intrinsic and extrinsic pathways. Direct binding assay by surface plasmon resonance m
The artificial chromosome represents a useful tool for gene transfer, both as cloning vectors and in chromosome biology research. To generate a Plasmodium artificial chromosome (PAC), we had to first functionally identify and characterize the parasite's centromere. A putative centromere (pbcen5) was cloned from chromosome 5 of the rodent parasite P. berghei based on a Plasmodium gene-synteny map. Plasmids containing pbcen5 were stably maintained in parasites during a blood-stage infection with h
