The University of Osaka · 생화학·유전·분자생물학
타카유키 카토 교수의 연구실은 주로 전자현미경 기반 고해상도 구조생물학을 중심으로, DNA 나노구조물과 박테리아의 운동 기구인 파라미터플라그렐루의 고해상도 구조를 규명하는 데 집중하고 있습니다. 특히 냉각전자현미경(cryo-EM)을 활용해 3.5Å 이하의 해상도로 단백질 복합체의 원자 구조를 해석함으로써, 구조적 안정성과 기능적 메커니즘을 밝혀내고 있습니다. 연구는 주로 박테리아의 운동성과 유전자 발현 전환 메커니즘, 그리고 나노스케일 구조물의 정밀한 설계 원리를 규명하는 데 초점을 맞추고 있습니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Many DNA nanostructures have been produced and a wide range of potential applications have been proposed. However, confirmation of accurate 3D construction is particularly challenging. Here, we demonstrate that cryoEM may be exploited to obtain structural information at sufficient resolution to visualize the DNA helix and reveal the absolute stereochemistry of a 7 nm self-assembled DNA tetrahedron. Structural analysis at such high resolution by cryoEM image analysis is unprecedented for any biol
The bacterial flagellum is a motility organelle consisting of a long helical filament as a propeller and a rotary motor that drives rapid filament rotation to produce thrust. <i>Salmonella</i><i>enterica</i> serovar Typhimurium has two genes of flagellin, <i>fljB</i> and <i>fliC</i>, for flagellar filament formation and autonomously switches their expression at a frequency of 10<sup>-3</sup>-10<sup>-4</sup> per cell per generation. We report here differences in their structures and motility func
Journal Article CryoTEM with a Cold Field Emission Gun That Moves Structural Biology into a New Stage Get access Takayuki Kato, Takayuki Kato Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan Search for other works by this author on: Oxford Academic Google Scholar Fumiaki Makino, Fumiaki Makino Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan Search for other works by this author on: Oxford Academic Google Scholar Takanori Nakane, Takano
The basal body of the bacterial flagellum is a rotary motor that consists of several rings (C, MS and LP) and a rod. The LP ring acts as a bushing supporting the distal rod for its rapid and stable rotation without much friction. Here, we use electron cryomicroscopy to describe the LP ring structure around the rod, at 3.5 Å resolution, from Salmonella Typhimurium. The structure shows 26-fold rotational symmetry and intricate intersubunit interactions of each subunit with up to six partners, whic
The Bacterial flagellar hook is a short supercoiled tubular structure made from a helical assembly of the hook protein FlgE. The hook acts as a universal joint that connects the flagellar basal body and filament, and smoothly transmits torque generated by the rotary motor to the helical filament propeller. In peritrichously flagellated bacteria, the hook allows the filaments to form a bundle behind the cell for swimming, and for the bundle to fall apart for tumbling. Here we report a native supe
The bacterial flagellum is a motility organelle consisting of a rotary motor and a long helical filament as a propeller. The flagellar hook is a flexible universal joint that transmits motor torque to the filament in its various orientations that change dynamically between swimming and tumbling of the cell upon switching the motor rotation for chemotaxis. Although the structures of the hook and hook protein FlgE from different bacterial species have been studied, the structure of <i>Salmonella</
