Kyoto University · 생화학·유전·분자생물학
Yoshihiro Sasaki 교수의 연구실은 주로 나노구조 수분체(나노젤)를 핵심으로 하여 의료용 나노소재 개발에 초점을 맞추고 있습니다. 단백질 약물 전달, 줄기세포 치료, 생체 영상 진단 등 신진대사 및 세포 기반 치료 기술의 핵심인 나노젤 기반 나노운반체 시스템을 개발하고 있으며, 특히 자기장 유도를 통한 정밀한 세포 내 전달 기술과 인공 쇼크프로테인 기능을 갖춘 나노젤을 통해 단백질의 안정성과 기능 회복을 향상시키는 데 기여하고 있습니다. 또한, 세포 외소체와의 하이브리드 시스템을 활용한 고감도 생체 신호 감지 기술 개발도 진행 중입니다.
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
Nanosize hydrogels (nanogels) are polymer nanoparticles with three-dimensional networks, formed by chemical and/or physical cross-linking of polymer chains. Recently, various nanogels have been designed, with a particular focus on biomedical applications. In this review, we describe recent progress in the synthesis of nanogels and nanogel-integrated hydrogels (nanogel cross-linked gels) for drug-delivery systems (DDS), regenerative medicine, and bioimaging. We also discuss chaperone-like functio
Protein pharmaceuticals show great therapeutic promise, but effective intracellular delivery remains challenging. To address the need for efficient protein transduction systems, we used a magnetic nanogel chaperone (MC): a hybrid of a polysaccharide nanogel, a protein carrier with molecular chaperone-like properties, and iron oxide nanoparticles, enabling magnetically guided delivery. The MC complexed with model proteins, such as BSA and insulin, and was not cytotoxic. Cargo proteins were delive
Abstract Nanogels are polymer nanoparticles with three-dimensional networks. Recently, various nanogels have been designed, with a particular focus on biomedical applications. In this review, we describe recent progress in the synthesis of functional nanogels by self-assembly of associating polymers and nanogel engineering for advanced biomedical technology including regenerative medicine and drug delivery systems.
Various cells in vivo secrete exosomes consisting of lipid bilayers. They carry mRNAs and miRNAs capable of controlling cellular functions and can be used as drug delivery system nanocarriers. There is the current need to further improve the efficiency of exosome uptake into target cells. In this study, we prepared a hybrid of exosomes and magnetic nanoparticles, which could be guided to target cells by a magnetic field for efficient uptake. Magnetic nanogels were prepared and hybridized to fluo
Cell-free protein synthesis is a promising technique for the rapid production of proteins. However, the application of the cell-free systems requires the development of an artificial chaperone that prevents aggregation of the protein and supports its correct folding. Here, nanogel-based artificial chaperones are introduced that improve the folding efficiency of rhodanese produced in cell-free systems. Although rhodanese suffers from rapid aggregation, rhodanese was successfully expressed in the
This paper describes construction of a nanosensory device for amplified detection of biologically important amines as chemical signals. The device was inspired by a biological signal transduction system, and was fabricated on an artificial cell membrane through self-organization of the molecular components, such as a synthetic receptor and a natural enzyme. Selective recognition of biologically important amines was achieved by a synthetic receptor with a pyridoxal moiety, as evaluated by means o
