慶應義塾大学 · 医学
Akiyoshi Hirayama教授の研究室では、がんや代謝疾患のメタボロミクス解析を目的として、高感度で高スループットな代謝物分析技術の開発と応用に取り組んでいます。特に、キャピラリー電気泳動と質量分析を組み合わせたCE-MS技術を応用し、臨床組織や血液サンプル中の Charged 代謝物を網羅的かつ定量的に解析しています。その成果として、がんのエネルギー代謝異常（ウォルバーグ効果）の解明や、臨床的バイオマーカーの同定が進められています。
Figures are computed from collected data and may differ slightly.
Most cancer cells predominantly produce energy by glycolysis rather than oxidative phosphorylation via the tricarboxylic acid (TCA) cycle, even in the presence of an adequate oxygen supply (Warburg effect). However, little has been reported regarding the direct measurements of global metabolites in clinical tumor tissues. Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged metabolites, to simultaneously mea
The development of high-throughput metabolite measurement technologies has enabled the use of metabolomics for epidemiologic studies by profiling metabolite concentrations in large cohorts of human blood samples. Standard protocols are necessary to obtain unbiased profiles through multiple runs over long periods of time and to allow reliable statistical analyses. This study assessed the effects of sampling procedures and storage conditions on the stability of metabolomic profiles in plasma and s
Sheath-flow capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a new tool for comprehensive analysis of charged metabolites. However, it needs to be more sensitive. Here, we report a sheathless capillary electrophoresis-electrospray ionization-mass spectrometry method for cationic metabolome analysis. This system used a high-sensitivity porous sprayer interface and 10% (v/v) acetic acid as the background electrolyte (BGE). Under optimized conditions, 53 cationic metabolites, incl
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