Daisuke Ino

Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIA_OT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for

Schwann cells (SCs) myelinate peripheral neurons to promote the rapid conduction of action potentials, and the process of myelination is known to be regulated by signals from axons to SCs. Given that SC mitochondria are one of the potential regulators of myelination, we investigated whether SC mitochondria are regulated by axonal signaling. Here, we show a purinergic mechanism that sends information from neurons to SC mitochondria during myelination. Our results show that electrical stimulation

The femtosecond time evolutions of excited states in zinc phthalocyanine (ZnPC) films and at the interface with TiO2(110) have been studied by using time-resolved two-photon photoelectron spectroscopy (TR-2PPE). The excited states are prepared in the first singlet excited state (S1) with excess vibrational energy. Two different films are examined: ultrathin (monolayer) and thick films of approximately 30 A in thickness. The decay behavior depends on the thickness of the film. In the case of the

The electronic structures of tris-(8-hydroxyquinoline) aluminum $(\mathrm{Al}{\mathrm{q}}_{3})$ on Cu(111) and Au(111) surfaces are studied by using ultraviolet photoelectron spectroscopy and two-photon photoelectron (2PPE) spectroscopy. The work function decreases with increase of the coverage due to surface dipole of 5.1 D along the surface normal. The ionization potential from the highest occupied state 6.38 eV does not depend on the metal substrates used in this study. The anion states of $\

The electronic excitations induced with tunneling electrons into adlayers of 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA) on Ag(111) have been investigated by in situ fluorescence spectroscopy in scanning tunneling microscopy (STM). A minute area of the surface is excited by an electron tunneling process in STM. Fluorescence spectra strongly depend on the coverage of PTCDA on Ag(111). The adsorption of the first PTCDA layer quenches the intrinsic surface plasmon originated from the clean

Facial expressions are widely recognized as universal indicators of underlying internal states in most species of animals, thereby presenting as a non-invasive measure for assessing physical and mental conditions. Despite the advancement of artificial intelligence-assisted tools for automated analysis of voluminous facial expression data in human subjects, the corresponding tools for mice still remain limited so far. Considering that mice are the most prevalent model animals for studying human h

ABSTRACT Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo . Here, we describe MTRIA OT , a G protein-coupled receptor-based green fluorescent OT sensor with a large dynamic range, optimal affinity, ligand specificity to OT or

The formation of the myelin sheath by Schwann cells (SCs) is essential for rapid conduction of nerve impulses along axons in the peripheral nervous system. SC-selective genetic manipulation in living animals is a powerful technique for studying the molecular and cellular mechanisms of SC myelination and demyelination in vivo. While knockout/knockin and transgenic mice are powerful tools for studying SC biology, these methods are costly and time consuming. Viral vector-mediated transgene introduc

Hearing loss is a pivotal risk factor for dementia. It has recently emerged that a disruption in the intercommunication between the cochlea and brain is a key process in the initiation and progression of this disease. However, whether the cochlear properties can be influenced by pathological signals associated with dementia remains unclear. In this study, using a mouse model of Alzheimer's disease (AD), we investigated the impacts of the AD-like amyloid β (Aβ) pathology in the brain on the cochl

Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe a G protein-coupled receptor-based green fluorescent OT sensor with a large dynamic range, optimal affinity, ligand specificity to OT orthologs, minimal effe

ABSTRACT Facial expressions are widely recognized as universal indicators of underlying internal states in most species of animals, thereby presenting as a non-invasive measure for predicting physical and mental conditions. Despite the advancement of artificial intelligence-assisted tools for automated analysis of voluminous facial expression data in human subjects, the corresponding tools for mice still remain limited so far. Considering that mice are the most prevalent model animals for studyi

The codes for the behavioral analyses used in K. Nitahara et al., Defective ventral neurogenesis due to midfetal Chd8 mutation drives autistic-like behavior in mice. Nature Commun. (2026)