東京大学 · 医学
黒田大輔教授の研究室では、がん患者の栄養状態を評価する新規指標「CONUT」の臨床的応用や、がん治療における次世代抗体薬の開発を支援するコンピュータ支援抗体モデリング技術の開発を進めています。特に、抗原認識の中心を占めるCDR-H3領域の構造予測に注力し、その多様性を解明するための分類法の刷新や、抗体の構造と機能の関係を解明する研究が特徴です。また、生体適合性に優れた高窒素ステンレス鋼の腐食抵抗性評価など、医療材料の開発にも取り組んでいます。
Figures are computed from collected data and may differ slightly.
CONUT is useful for not only estimating nutritional status but also for predicting long-term OS in gastric cancer patients after curative resection.
Recent clinical trials using antibodies with low toxicity and high efficiency have raised expectations for the development of next-generation protein therapeutics. However, the process of obtaining therapeutic antibodies remains time consuming and empirical. This review summarizes recent progresses in the field of computer-aided antibody development mainly focusing on antibody modeling, which is divided essentially into two parts: (i) modeling the antigen-binding site, also called the complement
Among the six complementarity-determining regions (CDRs) in the variable domains of an antibody, the third CDR of the heavy chain (CDR-H3), which lies in the center of the antigen-binding site, plays a particularly important role in antigen recognition. CDR-H3 shows significant variability in its length, sequence, and structure. Although difficult, model building of this segment is the most critical step in antibody modeling. Since our first proposal of the "H3-rules," which classify CDR-H3 stru
Supplementary data are available at Bioinformatics online.
The corrosion resistance of the nickel-free high nitrogen austenitic stainless steel without manganese, Fe–23Cr–2Mo–1.5N (mass%) (HNS) as biomaterials, was evaluated by the polarization test in various electrolytes: 0.9%NaCl solution (saline), phosphate buffered saline (PBS(-)), Hanks’ solution (Hanks) and Eagle’s minimum essential medium (E-MEM). Conventional austenitic stainless steel, 316L, was also polarized for comparison. The both alloys were spontaneously passivated in all electrolytes. T
Antibody modeling is widely used for the analysis of antibody-antigen interactions and for the design of potent antibody drugs. The antibody combining site is composed of six complementarity determining regions (CDRs). The CDRs, except for CDR-H3, which is the most diverse CDR, form limited numbers of canonical structures, which can be identified from the amino acid sequences. A method to classify the CDR-H3 structure from its amino acid sequence was previously proposed. However, since those CDR
Ingots of ferritic stainless steels, Fe–24Cr and Fe–24Cr–2Mo in mass%, were worked to various dimensions for test specimens. Nitrogen was absorbed by the specimens in a furnace filled with nitrogen gas with a pressure of 101.3 kPa at 1473 K to develop a simple and convenient manufacturing process of nickel-free austenitic stainless steels. Changes in the mechanical properties of the alloys with nitrogen absorption treatment are discussed on the basis of the resultant microstructure. Ferritic Fe–
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