Kyung Hee University · 生化学・遺伝学・分子生物学
Professor Gi-Ja Lee's research lab specializes in the development of advanced electrochemical and immunochromatographic sensors for point-of-care diagnostics, with a focus on non-invasive biomarker detection in saliva. The lab pioneers innovative nanomaterial-based platforms—such as graphene oxide, carbon nanotubes, and Prussian blue—integrated with screen-printed electrodes and nanoparticle modifications to enable sensitive, selective, and rapid detection of disease-related analytes like uric acid, hydrogen peroxide, and pepsin. Their work emphasizes practical applications in clinical diagnostics, particularly for conditions such as laryngopharyngeal reflux and neurological disorders, by optimizing sample collection, sensor fabrication, and signal transduction. The lab also explores the electrochemical modulation of neurotransmitters, linking sensor technology to physiological mechanisms such as glutamate release in ischemic conditions.
Figures are computed from collected data and may differ slightly.
In this study, we introduce a uricase-immobilized paper (UOx--paper) integrated electrochemical sensor for detection of uric acid (UA) in saliva. The UOx was immobilized on the detection zone in the wax-patterned paper substrate. This UOx-paper was integrated with a Prussian blue--modified, screen-printed carbon electrode after electropolymerization of o-phenylenediamine to construct an electrochemical cell for small-volume (20 μL) of samples. First, we optimized the fabrication conditions of UO
In this study, we fabricated platinum nanoparticles (PtNP)-decorated, porous reduced graphene oxide (rGO)–carbon nanotube (CNT) nanocomposites on a PtNP-deposited screen-printed carbon electrode (PtNP/rGO–CNT/PtNP/SPCE) for detection of hydrogen peroxide (H2O2), which is released from prostate cancer cells LNCaP. The PtNP/rGO–CNT/PtNP/SPCE was fabricated by a simple electrochemical deposition and co-reduction method. In addition, the amperometric response of the PtNP/rGO–CNT/PtNP/SPCE electrode
Salivary pepsin is a promising marker for the non-invasive diagnosis of laryngopharyngeal reflux (LPR). For reliable results regarding pepsin in saliva, it is critical to standardize the collection, storage, and pre-processing methods. In this study, we optimized the saliva collection protocols, including storage conditions, i.e., solution, temperature, and time, and the pre-processing filter for pepsin. Moreover, we prepared a simple immunochromatographic strip for the rapid detection of pepsin
This result suggests that the effect of acupuncture might be closely associated with modulation of the brain glutamate release in the ischemic condition.
Detection of salivary pepsin has been given attention as a new diagnostic tool for laryngopharyngeal reflux (LPR) disease, because saliva collection is non-invasive and relatively comfortable. In this study, we prepared polypyrrole nanocorals (PPNCs) on a screen-printed carbon electrode (SPCE) by a soft template synthesis method, using β-naphthalenesulfonic acid (NSA) (for short, PPNCs/SPCE). Gold nanoparticles (GNPs) were then decorated on PPNCs/SPCE by electrodeposition (for short, GNP/PP
This work describes the string sensor for the simple and sensitive detection of glucose which is based on Prussian blue (PB) modified graphite utilizing dipping. First, PB modified graphite (PB-G) strings are characterized by physical and electrochemical techniques to optimize the PB-G layer thickness. Then, glucose oxidase (GOx) is immobilized on PB-G string electrode with biocompatible chitosan overlayer (Chi/GOx/PB-G). The Chi/GOx/PB-G string electrode exhibits a sensitivity of 641.3 μ A·mM −
Hydrogen sulfide (H<sub>2</sub>S) is known to participate in bacteria-induced inflammatory response in periodontal diseases. Therefore, it is necessary to quantify H<sub>2</sub>S produced by oral bacteria for diagnosis and treatment of oral diseases including halitosis and periodontal disease. In this study, we introduce a paper-based colorimetric assay for detecting bacterial H<sub>2</sub>S utilizing silver/Nafion/polyvinylpyrrolidone membrane and a 96-well microplate. This H<sub>2</sub>S-sensi
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