北海道大学 · 医学
Hiroshi Nomoto教授の研究室では、糖鎖の構造と機能の解明を柱として、膜タンパク質に付加される糖鎖の生物学的役割を分子・細胞生物学的手法を用いて解明しています。特にトロペドー由来のアセチルコリン受容体をモデルとして、高マンノース型糖鎖の構造とその生理的意義をNMR分光法を用いて解析しています。また、ポリフェノール化合物の発がん予防作用に関する実験的評価も行い、がん予防における糖鎖と抗酸化物質の関連を解明しています。
Figures are computed from collected data and may differ slightly.
The structure of carbohydrates in acetylcholine receptor (AChR) from Torpedo californica is reported. Oligosaccharides released quantitatively from the whole molecule by N-oligosaccharide glycopeptidase digestion were fractionated by thin-layer chromatography and further purified by high-performance liquid chromatography. We show that more than 70% of the total oligosaccharide chains in Torpedo AChR are of the high-mannose type with the structures (Man)8(GlcNAc)2 and (Man)9(GlcNAc)2. The structu
Effects of proanthocyanidin (PA), procyanidin B-2 (B-2), and epigallocatechin gallate (EGCG) on azoxymethane (AOM)-induced colonic preneoplastic aberrant crypt foci (ACF) formation were investigated using F344 rats. The numbers of total ACF in rats treated with 0.002% PA and 0.05% B-2 were significantly decreased compared with the AOM alone group (control). Cell proliferation in the colon, as shown by proliferating cells nuclear antigen (PCNA), was also reduced in those treatments. The single-st
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