九州大学 · 医学
伊島弘行教授の研究室は、再生医療を基盤とした人工肝臓支援システムの開発を主眼としています。特に、多孔質フォームを用いた肝細胞スフィアロイドの形成とその機能的維持、ならびに脱細胞化肝マトリックス（L-ECM）を用いた肝細胞の長期的培養・機能維持の技術開発が進んでいます。これらの技術を応用し、肝不全モデルラットにおける生存率向上を実現する実用的で効果的なハイブリッド人工肝支援システムの構築を目指しています。
Figures are computed from collected data and may differ slightly.
Spherical multicellular aggregates (spheroids) of adult rat hepatocytes were spontaneously formed in the pores of polyurethane foam (PUF) as a culture substratum. Highly differentiated functions of hepatocytes, that is, albumin secretion, urea synthesis, and drug metabolism, were maintained in the spheroid culture using PUF. These spheroids were partly attached and immobilized in the pores of PUF. Then, we designed a PUF/spheroids packed-bed module as a hybrid artificial liver and developed an a
Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum. These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial live support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module includin
The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical pr
The extracellular matrix (ECM) in a liver-specific extracellular matrix (L-ECM) scaffold facilitates hepatocyte viability and maintains hepatocyte functions <i>in vitro</i>. However, whether an intact composition of ECM is required for an efficient ECM-based substrate design remains to be clarified. In this study, two L-ECM hydrogels, namely L-ECM I and L-ECM II, were prepared by pepsin solubilization at 4 °C and 25 °C, respectively. The solubility at 4 °C was 50% whereas that at 25 °C was 95%,
The hep-col scaffold can localize several kinds of growth factors as well as stabilize bFGF under physiological temperature and is a promising potent scaffold for regenerative medicine.
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