Tohoku University · Engineering
히토시 쇼쿠 교수가 이끄는 연구실은 주로 스캐닝 전기화학 현미경(SECM)을 핵심 기술로 활용하여 생체 분자 및 생체막의 국소적 전기화학적 특성을 정량적으로 분석하는 데에 집중하고 있습니다. 특히 배아의 산소 소비 측정, 효소 패턴 형성, 고분자막의 기질 전이 거동 등 생물학적 시스템에서의 물질 이동과 반응 메커니즘을 전기화학적 방법으로 규명하고 있습니다. 연구는 생체모사 장치, 생체 센서, 나노패터닝 기술 등 응용 분야로 확장되고 있습니다.
Figures are computed from collected data and may differ slightly.
Oxygen consumption of individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). A probe microelectrode was used to scan near a single embryo surface in a culture medium to monitor the oxygen reduction current at 37 degrees C, under a water-saturated atmosphere of 5% CO2 and 95% air. The oxygen concentration profiles near the embryos were in good agreement with the theoretical spherical diffusion. When an embryo reached the stage of a morula with a 74-m
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMicrofabrication and Characterization of Diaphorase-Patterned Surfaces by Scanning Electrochemical MicroscopyHitoshi. Shiku, Toshihiro. Takeda, Hiroshi. Yamada, Tomokazu. Matsue, and Isamu. UchidaCite this: Anal. Chem. 1995, 67, 2, 312–317Publication Date (Print):January 15, 1995Publication History Published online1 May 2002Published inissue 15 January 1995https://pubs.acs.org/doi/10.1021/ac00098a014https://doi.org/10.1021/ac00098a014research-articleAC
Abstract Scanning electrochemical microscopy (SECM) was employed to quantitatively characterize the oxygen permeation behaviors of poly(dimethylsiloxane) (PDMS) and surface-modified PDMS. The mass-transfer process of oxygen from the PDMS substrate to the tip electrode is diffusion limited, whereas the oxygen permeability of PDMS subjected to oxygen plasma treatment or albumin adsorption is critically restricted. Our results suggest that the oxygen permeability of PDMS is possibly affected by O2
Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼10(4) CEA molecules in a single 20-μm-radius spot.
Scanning electrochemical microscopy (SECM) was used for localized electrogeneration of hydroxyl radical to create patterns with diaphorase on the substrates immobilized with self-assembled monolayers (SAMs) of four different alkylsilane derivatives. Hydroxyl radical was generated at an SECM tip (microelectrode) in a solution containing H2O2 and Fe3+ by applying a reduction potential pulse of 0.00 V vs Ag/AgCl. The electrogenerated hydroxyl radical degraded the SAMs and removed them from the glas
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