Kazuhiko Nakatani

1. Membrane current was recorded from an isolated, dark-adapted toad rod by sucking either its inner segment or outer segment into a tight-fitting glass pipette containing Ringer solution. The remainder of the cell was exposed to bath solution which could be changed rapidly. 2. In normal Ringer solution the current response of a cell to a saturating flash or step of light showed a small secondary rise at its initial peak. The profile of this secondary rise (i.e. amplitude and time course) was in

The responses of rabbit rods to light were studied by drawing a single rod outer segment projecting from a small piece of retina into a glass pipette to record membrane current. The bath solution around the cells was maintained at near 40 degrees C. Light flashes evoked transient outward currents that saturated at up to approximately 20 pA. One absorbed photon produced a response of approximately 0.8 pA at peak. At the rising phase of the flash response, the relation between response amplitude a

2-Acylamino-1,8-naphthyridine (1), which possesses hydrogen bonding groups fully complementary to guanine (G), selectively binds to a single G bulge of duplex DNA. The melting temperature (Tm) of the duplex containing a G bulge was increased by the presence of 1, whereas no increase of Tm was observed for the duplexes containing adenine (A) and thymine (T) bulges as well as for normal duplex. Riboflavin-sensitized photooxidation of DNA containing GG steps opposite to G and A bulges was selective

1. The outer segment of an isolated rod photoreceptor from the bullfrog retina was drawn into a pipette containing choline solution for recording membrane current. The rest of the cell was sheared off with a glass probe to allow internal dialysis of the outer segment with a bath potassium solution ('truncated rod outer segment' preparation). The potential between the inside and the outside of the pipette was held at 0 mV. 2. Application of bath cGMP, in the presence of 3-isobutyl-1-methylxanthin

Dimeric 2-amino-1,8-naphthyridine selectively binds to a G−G mismatch with high affinity (Kd = 53 nM). We have investigated a binding mechanism of naphthyridine dimer 2 to a G−G mismatch by spectroscopic studies, thermodynamic analysis, and structure−activity studies for the thermal stabilization of the mismatch. 1H NMR spectra of a complex of 2 with 9-mer duplex d(CATCGGATG)2 containing a G−G mismatch showed that all hydrogens in two naphthyridine rings of 2 were observed upfield compared to th

1. In darkness, a single rod outer segment isolated from the toad retina was sucked partially, tip first, into a tight-fitting, Ringer solution-filled glass pipette for recording membrane current. The basal end of the outer segment outside the pipette was sheared off with a probe to allow internal dialysis. The potential between the inside and the outside of the pipette was held at 0 mV. 2. With cyclic GMP and IBMX (isobutylmethylxanthine) in the dialysis solution, a large inward current appeare

Technologies that discriminate between two alleles that differ from each other only in a single base sequence are needed for the typing of single-nucleotide polymorphisms (SNPs). Hybridization of probe DNAs to wild and mutant targets produces mismatched duplexes, whose chemical properties are clues in chemistry challenges for nonenzymatic SNP typing. Single nucleotide polymorphisms (SNPs) are gene variations that result from a single nucleotide difference.1–4 A single Watson–Crick base pair in w

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTChemistry of Sequence-Dependent Remote Guanine Oxidation: Photoreaction of Duplex DNA Containing Cyanobenzophenone-Substituted UridineKazuhiko Nakatani, Chikara Dohno, and Isao SaitoView Author Information Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University CREST, Japan Science and Technology Corporation (JST) Kyoto 606-8501, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 46, 10854–10855Publication

Recent reports have demonstrated that circulating endothelial cells (CECs) are observed in several diseases with vascular injury. Because Kawasaki disease (KD) is one type of systemic vasculitis, we hypothesized that an increased number of CECs may be associated with the appearance of complicated coronary artery lesions (CAL). In the present study we investigated the enumeration and origin of CECs in 20 patients with KD, using an immunohistochemical method with monoclonal antibodies: clone P1H12

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTModulation of DNA-Mediated Hole-Transport Efficiency by Changing Superexchange Electronic InteractionKazuhiko Nakatani, Chikara Dohno, and Isao SaitoView Author Information Department of Synthetic Chemistry and Biological Chemistry Faculty of Engineering, Kyoto University, CREST Japan Science and Technology Corporation (JST) Kyoto 606−8501, Japan Cite this: J. Am. Chem. Soc. 2000, 122, 24, 5893–5894Publication Date (Web):June 6, 2000Publication H

1. Membrane current was recorded from an isolated, dark-adapted salamander cone by sucking its inner segment into a tight-fitting glass pipette containing Ringer solution. The outer segment of the cell was exposed to a bath solution that could be changed rapidly. 2. After removing Na+ from the bath Ringer solution for a short period of time in darkness (the 'loading period'), a transient inward current was observed upon restoring it in bright light. A similar but longer-lasting current was obser