Kazuhiro Abe

ABSTRACT Ethylene had an undesirable effect on the quality of kiwifruits, bananas, broccoli, and spinach leaves that were prepared and stored as lightly processed products. A 2.or 20 ppm ethylene treatment hastened the softening of the pulp of kiwifruits and bananas held at 20°C. Use of charcoal with palladium chloride, as ethylene absorbent, prevented the accumulation of the ethylene and was effective in reducing the rate of softening in kiwifruits and bananas and of chlorophyll loss in spinach

It is generally known that spermatozoa aquire the capacity for fertilization during passage through the proximal region of the epididymal duct and are then stored in the distal region of the duct (BEDFORD, 1975; HAMILTON, 1975). The mouse epididymal duct has been divided into five segments (I-V) by light microscopy; Segments I, II and III constitute the head of the epididymis, Segment IV the body, and Segment V the tail; Segments I, II and III seem to belong to the proximal region, and Segments

Acid-related gastric diseases are associated with disorder of digestive tract acidification. The gastric proton pump, H(+),K(+)-ATPase, exports H(+) in exchange for luminal K(+) to generate a highly acidic environment in the stomach, and is a main target for acid suppressants. Here, we report the three-dimensional structure of gastric H(+),K(+)-ATPase with bound SCH28080, a representative K(+)-competitive acid blocker, at 7 Å resolution based on electron crystallography of two-dimensional crysta

ATP11C, a plasma membrane phospholipid flippase, maintains the asymmetric distribution of phosphatidylserine accumulated in the inner leaflet. Caspase-dependent inactivation of ATP11C is essential for an apoptotic "eat me" signal, phosphatidylserine exposure, which prompts phagocytes to engulf cells. We show six cryo-EM structures of ATP11C at 3.0-4.0 Å resolution in five different states of the transport cycle. A structural comparison reveals phosphorylation-driven domain movements coupled with

The blood supply, microvasculature, and ultrastructure of the capillaries in the epididymis in adult mice were regionally examined. The epididymal duct of the initial segment is surrounded with a dense network of fenestrated capillaries running just under the epithelium. The other segments have loose networks of nonfenestrated capillaries running in the interductal connective tissue. The fenestration of capillaries in the initial segment was markedly reduced in frequency immediately after cuttin

ATP11C, a member of the P4-ATPase flippase, translocates phosphatidylserine from the outer to the inner plasma membrane leaflet, and maintains the asymmetric distribution of phosphatidylserine in the living cell. We present the crystal structures of a human plasma membrane flippase, ATP11C-CDC50A complex, in a stabilized E2P conformation. The structure revealed a deep longitudinal crevice along transmembrane helices continuing from the cell surface to the phospholipid occlusion site in the middl

Peyer's patches of adult mice were morphologically studied by light microscopy.The patch is topographically divided into four portions: germinal center, follicular area, parafollicular area and dome area. The germinal center consists of light and dark zones. The follicular area surrounds the germinal center, especially the light zone which has an apical pole directed toward the mucosal surface. The parafollicular area surrounds the follicular area and contains postcapillary venules with high end

Two isozymes of gamma-glutamyltranspeptidase, GGT-A and GGT-B, were purified to electrophoretic homogeneity from a culture broth of Bacillus subtilis TAM-4, which produces poly(gamma-glutamic acid) (PGA) de novo. GGT-A was composed of three subunits with molecular weights of 23,000 (I), 39,000 (II), and 40,000 (III). GGT-B was composed of two subunits with molecular weight of 22,000 (I) and 39,000 (II). The N-terminal amino acid sequences of GGT-A subunit I and GGT-B subunit I were very similar.

Different parts of the epididymal duct were ligated when mice were 90 days old. The mice were killed 1--4 weeks later. PAS-positive materials appeared in the epithelial cells of Segment IV (corpus epididymidis) after ligation of the efferent ducts or at Segment II (middle part of caput) but not when the ligature was distal to Segment II. The inclusions were seen as early as 1 week after ligation and became increased in size and number with time.

Gastric H(+),K(+)-ATPase is responsible for gastric acid secretion. ATP-driven H(+) uptake into the stomach is efficiently accomplished by the exchange of an equal amount of K(+), resulting in a luminal pH close to 1. Because of the limited free energy available for ATP hydrolysis, the stoichiometry of transported cations is thought to vary from 2H(+)/2K(+) to 1H(+)/1K(+) per hydrolysis of one ATP molecule as the luminal pH decreases, although direct evidence for this hypothesis has remained elu

The efferent duct or the epididymal duct at the border between the caput and corpus epididymidis was cut or ligated in mice at the following stages: birth, 20 days, and 60 days of age. The epididymal duct of the corpus epididymidis was observed at 60 days of age after neonatal or juvenile operation and 4 weeks after the adult operation. After efferent duct interruption, the epididymal duct in the corpus possessed abundant PAS-positive material with no spermatozoa in the lumen, and the principal

Mouse epididymides were examined by light and electron microscopy 6, 12, 24 h, 1.5, 2, 3, and 5 days after efferent duct cutting when the mice were 60 days of age. Six hours after operation, the principal cells in the initial segment of the epididymal duct began to degenerate following the disappearance of intraluminal spermatozoa. The degenerated cells increased rapidly and reached their greatest number 24 h, and then decreased to smaller numbers until 48 h. These degenerative changes were foll