京都大学 · 生化学・遺伝学・分子生物学
Kiyoshi Yasukawa教授の研究室では、インターリーキン-6受容体（IL-6R）の機能解析と再結合体の精製・応用を柱とした細胞接着・シグナル伝達の分子機構を解明しています。特に、可溶性IL-6受容体（sIL-6R）の定量法開発や、その生物学的活性の評価を基盤に、炎症・自己免疫疾患のメカニズム解明に貢献しています。また、逆転錄酵素の耐熱性評価を通じて、分子生物学的技術の高度化にも取り組んでいます。
Figures are computed from collected data and may differ slightly.
An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sIL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1 cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recombinant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant sIL-6R augmented the sensi
Reverse transcriptases (RTs) from avian myeloblastosis virus (AMV) and Moloney murine leukaemia virus (MMLV) have been most extensively used as a tool for conversion of RNA to DNA. In this study, we compared the thermal stabilities of AMV RT and MMLV RT by observing their irreversible thermal inactivation. The temperatures reducing initial activity by 50% in 10-min incubation, T(50), of AMV RT were 47 degrees C without the template-primer (T/P), poly(rA)-p(dT)(12-18), and 52 degrees C with the T
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