大阪大学 · 生化学・遺伝学・分子生物学
本田耕介教授の研究室では、合成生物学的手法を応用したバイオケミカルプロセスの構築をめざしています。特に、体外で再構成可能な人工代謝経路の構築により、工業的有用な化学品を効率的に生産する新しいバイオ触媒技術の開発を進めています。高温に安定な酵素を組み合わせた人工オペロンを用いた一括発現技術により、複雑な代謝経路の簡素化と最適化を実現しています。
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In vitro reconstitution of an artificial metabolic pathway is an emerging approach for the biocatalytic production of industrial chemicals. However, several enzymes have to be separately prepared (and purified) for the construction of an in vitro metabolic pathway, thereby limiting the practical applicability of this approach. In this study, genes encoding the nine thermophilic enzymes involved in a non-ATP-forming chimeric glycolytic pathway were assembled in an artificial operon and co-express
Bio-based chemical production has drawn attention regarding the realization of a sustainable society. <i>In vitro</i> metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes <i>in vitro</i>. Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel
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