東京大学 · 生化学・遺伝学・分子生物学
田貫麻呂教授の研究室では、主に核磁気共鳴（NMR）分光法を応用し、生体分子の構造・動態およびその酸解離挙動を解明する研究を行っています。特に、ヒスチジンを含むペプチドのpKa値と化学シフトの関係をNMR滴定法で解析し、分子内相互作用の影響を定量的に評価しています。また、緑コーヒー抽出物のような複雑混合物の成分同定・定量に対しても、高次元NMR技術を駆使した分析手法の開発を進めています。
Figures are computed from collected data and may differ slightly.
Abstract The nmr titration curves of chemical shifts versus pH were observed for the protons of various histidine‐containing di‐ and tripeptides. With these results, the macroscopic p K a values and the chemical shifts intrinsic to each ionic species were determined by a computer curve‐fitting based on a simple acid dissociation sequence. The p K a value of the imidazole ring in N ‐acetyl‐ L ‐histidine methylamide was assumed to represent the intrinsic (or unperturbed) p K a of the imidazole rin
The binding of the chymotryptic troponin T subfragments to tropomyosin, troponin I, and troponin C was semiquantitatively examined by using affinity chromatography, and also by co-sedimentation with F-actin and polyacrylamide gel electrophoresis in 14 mM Tris/90 mM glycine. Circular dichroism spectra of the subfragments were measured to confirm that the subfragments retained their conformational structures. Based on these results, the binding sites of tropomyosin, troponin I, and troponin C on t
A complex mixture analysis by one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy was carried out for the first time for the identification and quantification of organic compounds in green coffee bean extract (GCBE). A combination of (1)H-(1)H DQF-COSY, (1)H-(13)C HSQC, and (1)H-(13)C CT-HMBC two-dimensional sequences was used, and 16 compounds were identified. In particular, three isomers of caffeoylquinic acid were identified in the complex mixture without any separation. In
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