北海道大学 · 生化学・遺伝学・分子生物学
山岸美鈴教授の研究室は、被子植物の花や葉に見られる色素の発現機構、特にアントシアニンとカロテノイドの生合成とその発現調節を分子遺伝学的・分子生物学的手法を用いて解明しています。特に、リュウキンソウ（アジアティックハイブリッドリュウキンソウ）をモデルとして、R2R3-MYB転写因子やミクロルナノRNA（miR828）が色素斑の形成に果たす役割を解明しています。また、栄養素の一つであるホウ素がマメ科植物の窒素固定に与える影響についても、生理的・分子的アプローチで研究を展開しています。
Figures are computed from collected data and may differ slightly.
Anthocyanins are secondary metabolites that contribute to colors of flowers, fruits and leaves. Asiatic hybrid lily (Lilium spp.) accumulates cyanidin anthocyanins in flower tepals, tepal spots and leaves of juvenile shoots. To clarify their mechanisms of regulation of anthocyanin pigmentation, two full-length cDNAs of R2R3-MYB (LhMYB6 and LhMYB12) were isolated from the anthocyanin-accumulating tepals of cultivar 'Montreux'. Analysis of the deduced amino acid sequences indicated they have homol
With 6 figures and 2 tables To elucidate the regulatory mechanisms of carotenoid accumulation in tepals of Asiatic hybrid lily (Lilium spp.), the content and composition of carotenoids and transcriptional changes of carotenoid biosynthetic genes were compared between tepals of yellow, red, pink and white flowers. Yellow and red tepal cultivars accumulated high amounts of carotenoids, and cultivars with pink and white tepals accumulated small amounts of carotenoids. Most of the carotenoids in yel
Many angiosperm families develop spatially regulated anthocyanin spots on their flowers. The Asiatic hybrid lily (Lilium spp.) cv 'Latvia' develops splatter-type spots on its tepals. The splatters arise simply from the deposition of anthocyanin pigments in the tepal epidermis. To determine how splatter development was regulated, we analysed the transcription of anthocyanin biosynthesis genes, and isolated and characterized an R2R3-MYB gene specific to splatter pigmentation. All anthocyanin biosy
Abstract Effects of boron (B) on soybean growth, nodule development, and nitrogen fixation were studied. When soybean plants were grown in B-free medium for 35,40, or 72 d, B deficiency symptoms appeared. Nodules of B-deficient soybeans were damaged and showed low acetylene reduction activities. Soybean seed production was seriously depressed. Production of whole plant, nodules and pods was maximum at concentrations of 15–180 μg B L-1 (1.4–16 μm). At concentrations of 29–88 μg B L-1 nitrogen fix
To examine whether parental alleles were randomly transmitted from heterozygous donor plants to anther culture (AC)-derived plants, two AC-derived populations and one F2 population in rice were compared for 50 RFLP markers distributed on the rice chromosomes. Two populations which were developed through different anther culture methods (the ordinary method and the direct regeneration method) and the F2 population were produced from an F1 hybrid between distantly related cultivars of Nipponbare (
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