Naoki Komatsu

Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have shed new light on the spatiotemporal dynamics of signaling molecules. Among them, intramolecular FRET biosensors have been increasingly used due to their high sensitivity and user-friendliness. Time-consuming optimizations by trial and error, however, obstructed the development of intramolecular FRET biosensors. Here we report an optimized backbone for rapid development of highly sensitive intram

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic asymmetric oxidation of sulfides to sulfoxides with tert-butyl hydroperoxide using binaphthol as a chiral auxiliaryNaoki Komatsu, Masaya Hashizume, Toshio Sugita, and Sakae UemuraCite this: J. Org. Chem. 1993, 58, 17, 4529–4533Publication Date (Print):August 1, 1993Publication History Published online1 May 2002Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/jo00069a009https://doi.org/10.1021/jo00069a009research-articleACS Publ

Making even tiny diamonds valuable: Nanodiamonds (NDs) were covalently functionalized with hyperbranched polyglycerol (PG) by ring-opening polymerization to improve their suitability for biomedical applications (see scheme). The PG-grafted NDs were highly soluble not only in pure water (>20 mg mL−1), but also in a buffer (>16 mg mL−1), and were separated according to size by size-exclusion chromatography.

Upon contact with biofluids, proteins are quickly adsorbed onto the nanoparticle (NP) surface to form a protein corona, which initiates the opsonization and facilitates the rapid clearance of the NP by macrophage uptake. Although polyethylene glycol (PEG) functionalization has been the standard approach to evade macrophage uptake by reducing protein adsorption, it cannot fully eliminate nonspecific uptake. Herein, polyglycerol (PG) grafting is demonstrated as a better alternative to PEG. NPs of

Nanodiamond(ND)-based technologies are flourishing in a wide variety of fields spanning from electronics and optics to biomedicine. NDs are considered a family of nanomaterials with an sp³ carbon core and a variety of sizes, shapes, and surfaces. They show interesting physicochemical properties such as hardness, stiffness, and chemical stability. Additionally, they can undergo ad-hoc core and surface functionalization, which tailors them for the desired applications. Noteworthy, the p

In the field of nanomedicine, nanoparticles with various functions are required for in vivo applications such as biomedical imaging and drug delivery. Therefore, chemical functionalization of nanoparticles has been extensively investigated. Herein, nanodiamond (ND) coated with polyglycerol (PG) and its derivatives is reported to impart good solubility in a physiological environment, a stealth nature to avoid nonspecific uptake, a targeting property to be taken up by a specific cell, and an acid‐

Current chemotherapy of advanced non-small cell lung cancer (NSCLC) produces only a modest increase in survival time. New approaches are needed for this disease. The development of lung cancer is associated with silencing tumor suppressor genes that can occur not only by deletion or mutation, but also by epigenetic changes including histone deacetylation of key lysines. Histone deacetylase inhibitor (HDACI) increases histone acetylation, resulting in DNA with a more open chromatin that favors tr

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetic resolution of sulfoxides catalyzed by chiral titanium-binaphthol complexNaoki Komatsu, Masaya Hashizume, Toshio Sugita, and Sakae UemuraCite this: J. Org. Chem. 1993, 58, 26, 7624–7626Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/jo00078a060https://doi.org/10.1021/jo00078a060research-articleACS PublicationsRequest reuse permissionsArticle

Structural control of single-walled carbon nanotubes (SWNTs) is attracting enormous interest in view of their applications to nanoelectronics and nanooptics. Actually, more than 200 papers regarding separation of SWNTs have been published since 1998. In this review, they are classified into the following five sections according to the separation methods; electrophoresis, centrifugation, chromatography, selective solubilization and selective reaction. In each method, all literature is summarized

Abstract For biomedical application of nanoparticles, the surface chemical functionality is very important to impart additional functions, such as solubility and stability in a physiological environment, and targeting specificity as an imaging probe and a drug carrier. Although polyethylene glycol (PEG) has been used extensively, here, it is proposed that hyperbranched polyglycerol (PG) is a good or even better alternative to PEG. Superparamagnetic iron oxide nanoparticles (SPIONs) prepared usin

Nanodiamond (ND) particles as small as 4 nm are separated from powdered ND prepared by a static high-pressure high-temperature (HPHT) method using ultracentrifugation. The size of the isolated ND is tunable, ranging from 4 to 25 nm, by the selection of appropriate duration and acceleration of the centrifugation. The availability of various sizes of ND particles enables full and precise investigation of their size effects.

Genetically encoded biosensors based on the principle of Förster resonance energy transfer comprise two major classes: biosensors based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET). The FRET biosensors visualize signaling-molecule activity in cells or tissues with high resolution. Meanwhile, due to the low background signal, the BRET biosensors are primarily used in drug screening. Here, we report a protocol to transform intramolecula