九州大学 · 生化学・遺伝学・分子生物学
Noriho Kamiya教授の研究室では、微生物トランスグルタミナーゼを用いたタンパク質の特異的結合技術や、酵素触媒を用いたエナンチオ選択性反応の開発を柱としています。特にN末端グリシンを標的としたタンパク質・DNAのサイトスペシフィックな結合技術や、生体適合性ハイドロゲルの構築による細胞封入・リリース技術の開発が進んでいます。また、非イオン界面活性剤コーティングによる酵素活性の向上も、バイオケミストリー的応用の基盤として重要です。
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Here, we report the N-terminal glycine (Gly) residue of a target protein can be a candidate primary amine for site-specific protein conjugation catalyzed by microbial transglutaminase (MTG) from Streptomyces mobaraensis. Gly5-enhanced green fluorescent protein (EGFP) (EGFP with five additional Gly residues at its N-terminus) was cross-linked with Myc-dihydrofolate reductase (DHFR) (DHFR with the myc epitope sequence at its N-terminus) to yield DHFR-EGFP heterodimers. The reactivities of addition
Abstract Enantioselective esterification of menthol with fatty acids using a surfactant‐coated lipase was carried out in organic media. The surfactant‐coated lipase originating from Candida cylindracea appeared to be highly enantioselective and good biocatalyst for the resolution of racemic menthol. The enzymatic activity of the lipase in organic media was significantly increased by a coating with a nonionic surfactant. The reaction rate of the coated lipase was more than 100 times that of the p
DNA was site-specifically conjugated to a substrate peptide of microbial transglutaminase fused to the N- or C-terminus of target proteins without the loss of the proteins' functions of interest.
Horseradish peroxidase-mediated oxidative cross-linking of a thiolated poly(ethylene glycol) is promoted in the absence of exogenous hydrogen peroxide, by adding a small amount of a phenolic compound under physiological conditions. The prepared hydrogel can encapsulate and release living mammalian cells.
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