Hokkaido University · Biochemistry, Genetics and Molecular Biology
수평휘 교수의 연구실은 지질 대사 및 지질학적 변화가 질병 발생에 미치는 영향을 중심으로 연구를 진행하고 있습니다. 특히 리포프로테인, 플라스말로겐, 스피링오신-1-인산 등 생활화학적 지질 분자의 기능과 산화 스트레스, 지방세포 축적과 관련된 병리적 메커니즘을 분석하고 있습니다. 고감도 질량분석 기반의 지질체학적 분석 기법 개발을 통해 임상 생체류체 및 세포 내 지질 프로파일을 정밀하게 분석하는 데도 기여하고 있습니다.
Figures are computed from collected data and may differ slightly.
The physiological functions of lysophosphatidylethanolamine (lysoPE) have not been fully elucidated. In this study, the effects of lysoPE on lipogenesis and lipolysis were investigated in a cultured human liver-derived cell line. The intracellular lipid profile was investigated in detail using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to better understand the underlying mechanism. The expression of genes related to lipid metabolism and catabolism was analyzed using real-time PCR.
Plasmalogens derived from dietary phospholipids are considered to be potential protectors against oxidation-related disorders, while lead (Pb) is an environmental contaminant worldwide and is known to induce oxidative stress. However, the protective and antilipid oxidative effects of individual plasmalogen species against Pb damage have received little attention. In this study, six plasmalogen species (with either choline or ethanolamine as the headgroup and p16:0/18:1, p16:0/18:2, or p16:0/20:5
Sphingosine-1-phosphate (S1P) is a bioactive mediator in inflammation. Dysregulated S1P is demonstrated as a cause of heart failure (HF). However, the time-dependent and integrative role of S1P interaction with receptors in HF is unclear after myocardial infarction (MI). In this study, the sphingolipid mediators were quantified in ischemic human hearts. We also measured the time kinetics of these mediators post-MI in murine spleen and heart as an integrative approach to understand the interactio
This developed liquid chromatography-mass spectrometry method is convenient and reliable, which might be useful for monitoring the variations of short-chain fatty acids in blood.
Chronic kidney disease (CKD), which is defined as a condition causing the gradual loss of kidney function, shows renal lipid droplet (LD) accumulation that is associated with oxidative damage. There is a possibility that an LD abnormality in quality plays a role in CKD development. This study aimed to explore the chemical composition of LDs that are induced in human kidney cells during exposure to free fatty acids as an LD source and oxidized lipoproteins as oxidative stress. The LDs were aspira
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