Seoul National University · 農学・生物学
Professor Si Hyeock Lee's research lab focuses on insect molecular biology and neurotoxicology, with a primary emphasis on acetylcholinesterases (AChEs) and voltage-sensitive sodium channels in insects. The lab investigates the molecular mechanisms underlying insect neurophysiology, pesticide resistance, and cholinesterase function, particularly in agriculturally and ecologically significant species such as honey bees, the Colorado potato beetle, and the pinewood nematode. Their work combines molecular biology, biochemistry, and functional genomics to understand gene expression, enzyme kinetics, and resistance mechanisms at the genetic and physiological levels.
Figures are computed from collected data and may differ slightly.
We investigated the molecular and kinetic properties of two acetylcholinesterases (AmAChE1 and AmAChE2) from the Western honey bee, Apis mellifera. Western blot analysis revealed that AmAChE2 has most of catalytic activity rather than AmAChE1, further suggesting that AmAChE2 is responsible for synaptic transmission in A. mellifera, in contrast to most other insects. AmAChE2 was predominately expressed in the ganglia and head containing the central nervous system (CNS), while AmAChE1 was abundant
Resistance to the rapid paralytic effects of pyrethroids in the permethrin-resistant (PE-R) strain of the Colorado potato beetle (CPB) is phenotypically similar to that of thekdrtrait of the house fly, which is caused by a single amino acid substitution (L1014F) in theVssc1voltage-sensitive sodium channel gene of that species. To test the hypothesis that thekdr-like trait of the PE-R strain of the CPB is caused by a similar mechanism, we amplified a 370-bp segment of the CPB ortholog of the hous
The pinewood nematode genome encodes at least three distinct acetylcholinesterases (AChEs). To understand physiological roles of the three pinewood nematode AChEs (BxACE-1, BxACE-2, and BxACE-3), BxACE-3 in particular, their tissue distribution and inhibition profiles were investigated. Immunohistochemistry revealed that BxACE-1 and BxACE-2 were distributed in neuronal tissues. In contrast, BxACE-3 was detected from some specific tissues and extracted without the aid of detergent, suggesting its
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