Sunghoon Park
Ulsan National Institute of Science and Technology 화학과 · 生化学・遺伝学・分子生物学
Sunghoon Park 교수의 연구실은 메탄오자산균(Methylosinus trichosporium OB3b)과 대장균을 이용한 생물공정 최적화를 핵심으로 하며, 특히 효소(MMO, pMMO, sMMO)의 발현 조절과 재조조합 단백질 생산의 안정성 및 생산성을 극대화하는 데 초점을 맞추고 있습니다. 구체적으로, 구리 농도, 온도, 배지 조성, 배양 조건 등을 제어하여 생물학적 생산 공정의 효율성을 높이는 데 기여하고 있으며, 플라스미드 안정성과 유전자 발현 조절 전략 개발도 중요한 연구 분야입니다. 이는 바이오연료, 생물정화, 고부가가치 화학물질 생산 등 응용 분야로 이어지는 기초 연구입니다.
Figures are computed from collected data and may differ slightly.
The obligatory methanotroph, Methylosinus trichosporium OB3b, was studied to optimize the batch culture conditions for the formation of particulate methane monooxygenase (pMMO) in a nitrate minimal salts medium. The important medium components investigated were copper, carbon dioxide, and nitrate. The whole-cell specific pMMO activity decreased sharply with increasing copper concentrations in the range of 10-40 microM and remained constant upon further increases of the copper concentration to 12
Methanotrophs have promising applications in bioremediation and in the production of fuel-related chemicals due to their nonspecific enzyme, methane monooxygenase (MMO). The optimal conditions for cell growth and production of the soluble from of MMO (sMMO) were determined from batch cultivations of an obligatory methanotrophs, Methylosinus trichosporium OB3b, in shake flasks and a 5-L bioreactor. It was confirmed that a copper deficiency is essential for the formation of the cytoplasmic sMNO. O
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