大阪大学 · 生化学・遺伝学・分子生物学
Yoshie Harada教授の研究室では、細胞内での熱伝導メカニズムやバイオマクロレールの分子運動を、ナノスケールの熱・光デバイスを用いて高精度に解明しています。特に、蛍光ナノダイヤモンドを用いたナノヒーター・ナノサーモメーターを細胞内に組み込み、細胞内の局所的熱伝導率を測定する画期的な手法を開発。また、アクチン-ミオシン系のリアルタイム観察や、GC-rich配列を特異的に認識するポリアミドの設計・評価を通じて、細胞機能制御のための分子工学的アプローチを展開しています。
Figures are computed from collected data and may differ slightly.
Understanding heat dissipation processes at nanoscale during cellular thermogenesis is essential to clarify the relationships between the heat and biological processes in cells and organisms. A key parameter determining the heat flux inside a cell is the local thermal conductivity, a factor poorly investigated both experimentally and theoretically. Here, using a nanoheater/nanothermometer hybrid made of a polydopamine encapsulating a fluorescent nanodiamond, we measured the intracellular thermal
We used video-fluorescence microscopy to directly observe the sliding movement of single fluorescently labeled actin filaments along myosin fixed on a glass surface. Single actin filaments labeled with phalloidin-tetramethyl-rhodamine, which stabilizes the filament structure of actin, could be seen very clearly and continuously for at least 60 min in 02-free solution, and the sensitivity was high enough to see very short actin filaments less than 40 nm long that contained less than eight dye mol
N-Methylpyrrole (Py)-N-methylimidazole (Im) polyamides are small organic molecules that can recognize predetermined DNA sequences with high sequence specificity. As many eukaryotic promoter regions contain highly GC-rich sequences, it is valuable to synthesize and characterize Py-Im polyamides that recognize GC-rich motifs. In this study, we synthesized four hairpin Py-Im polyamides 1-4, which recognize 5'-GCGC-3' and investigated their binding behavior with surface plasmon resonance assay. Py-I
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