유현주 교수

Glycosides are important functional materials infoods. Transformation, especially hydrolysis, of the sugarmoieties tends to improve the functional properties of theadministered glycosides in vivoand edible food-grade fungi such as bifidobacteria, lactobacilli,leuconostocs, yeasts, and aspergilli are potential industrialmicroorganisms to transform glycosides of ginsenosides fromginseng, platycodin saponins from Platycodon grandiflorum,Trignoella foenum-graecum (TFG) saponins, and isoflavonesfrom

Clostridium difficile infection (CDI) is one of the most common nosocomial infections. Dysbiosis of the gut microbiota due to consumption of antibiotics is a major contributor to CDI. Recently, fecal microbiota transplantation (FMT) has been applied to treat CDI. However, FMT has important limitations including uncontrolled exposure to pathogens and standardization issues. Therefore, it is necessary to evaluate alternative treatment methods, such as bacteriotherapy, as well as the mechanism thro

An Er:YAG laser with 2940-nm wavelength and 250-μs pulse duration is used to generate a microjet that is ejected at ∼50 m/s in air. The strength of the microjet depends on the bubble dynamics from the beam-water interaction within the driving chamber as well as the discharging of the drug solution underneath the elastic membrane that separates the drug from the driving liquid. The jet characteristics, such as velocity, volume, and level of atomization, are obtained by high-speed camera images ta

The polysaccharide (BB-pol) extracted from Bifidobacterium bifidum BGN4 showed growth inhibitory effects on several colon cancer cell lines such as HT-29 and HCT-116. To increase the yield of polysaccharide, B. bifidum BGN4 was cultured in various culture media with different compositions. When B. bifidum BGN4 was cultured in modified MRS broth containing phytic acid, the cells showed increased branch formation and enlarged morphology. The content of total carbohydrate and the ability of adhesio

The matrix metalloproteinases (MMP) play an important role in tumor invasion, angiogenesis and inflammatory tissue destruction. Increased expression of MMP was observed in benign tissue hyperplasia and in atherosclerotic lesions. Invasive cancer cells utilize MMP to degrade the extracellular matrix and vascular basement membrane during metastasis, where MMP-2 has been implicated in the development and dissemination of malignancies. The present study attempted to examine the antiangiogenic activi

Ginsenosides have been regarded as the principalcomponents responsible for the pharmacological and biologicalactivities of ginseng. The transformation of ginsenosides withRc into Rd, but the reactions were slow. When the crudeenzymes obtained from several lactic acid bacteria were usedfor transformation, those from Bifidobacterium sp. Int57exhibited the most potent transforming activity of ginsenosidesto compound K. In comparison, a relatively higher levelof Rh2 was produced by the enzymes from

A pyrotechnic device that consists of a donor/acceptor pair separated by a gap or a bulkhead relies on the shock attenuation characteristics of the gap material and the shock sensitivity of the donor and acceptor explosives. In this study, a miniaturized exploding foil initiator, based on high pulsed electrical power generator, was designed to launch a micro Kapton® flyer for impact initiation of a high explosive in order to understand its performance characteristics. Here, the explosive substan

In a new Tatiana‐2 mission the measurement of transient luminous events (TLE) in the Earth atmosphere in nadir direction are planned. Near UV temporal images of TLE in millisecond scale will be measured together with temporal profiles in 8 channels of wide spectrum of TLE emission. Simultaneously temporal variation of electron flux at the satellite orbit will be measured. Aims of these measurements are to continue research of bright UV flashes, started in the Tatiana‐1 mission (Universitetsky–Ta

이 연구는 직무요구-자원 모형(JD-R)을 적용하여 기업의 산업 특성을 고려한 다집단 경로분석을 통해 제조업과 비제조업 종사자의 조직신뢰, 위계적 조직문화, 직무소진, 직무몰입 및 이직의도의 경로를 구명하는 데 목적이 있다. 이를 위해 한국직업능력개발원의 인적자본 기업패널조사(HCCP) 자료 중 대졸 근로자(2017년 당시) 코호트를 제조업과 비제조업 근로자로 구분하고 다집단 경로분석을 실시하였다. 연구를 통해 도출된 결론은 다음과 같다. 첫째, 조직신뢰, 위계적 조직문화, 직무소진, 직무몰입 및 이직의도 모형은 적합도 검정 결과 모든 지수가 양호하게 도출되었으며 이는 각 변인간의 경로를 예측하기에 적합하다고 판단되었다. 둘째, 경로분석 결과 연구 대상 전체에 대한 조직신뢰, 위계적 조직문화, 직무소진, 직무몰입 및 이직의도 경로는 모두 유의하였다. 셋째, 다집단 경로분석 비교결과 제조업 대비 비제조업 근로자들은 조직신뢰가 직무소진에 미치는 부적인 영향력이 더 큰 것으로 나타났으며

In spite of the reported probiotic effects, Bifidobacterium bifidum BGN4 (BGN4) showed no β- glucosidase activity and failed to biotransform isoflavone glucosides into the more bioactive aglycones during soy milk fermentation. To develop an isoflavone-biotransforming BGN4, we constructed the recombinant B. bifidum BGN4 strain (B919G) by cloning the structural β- glucosidase gene from B. lactis AD011 (AD011) using the expression vector with the constitutively active promoter 919 from BGN4. As a r

Bifidobacterium longum RD47 was cultured in 24 kinds of modified MRS broths containing various ingredients to select the most promising source that induces microbial enzymes. Among the various ingredients, ascorbic acid significantly enhanced a-L-arabinofuranosidase and a-L-arabinopyranosidase activities in Bifidobacterium longum RD47. Addition of 2 % ascorbic acid (w/v) to MRS showed the maximum enzyme activities. Both whole cell and disrupted cell homogenates showed efficient q-nitrophenyl-b-D

A jet engine for high speed air breathing propulsion is subject to continuous wear as a result of impacts of micro-scale ice particles during a flight in the atmosphere. The inlet duct and compressor blades are exposed to on-coming frozen moisture particles that may result in the surface damage and significantly shorten the designed lifetime of the aircraft. Under such prolonged high-speed impact loading, the performance parameters such as flight instability and power loss of a jet engine can be

Background: Thromboelastography (TEG) provides comprehensive information on the whole blood clot formation phases, whereas thrombin generation assay (TGA) reveals the endogenous thrombin levels in plasma. We investigated the potential significance of TEG and TGA parameters for prediction of clinical bleeding in hematologic patients on the basis of the patient’s platelet levels. Methods: TEG and TGA were performed in 126 patients with thrombocytopenia or hematologic malignancies. The bleeding ten

Trigonella foenum-graecum, commonly known as fenugreek, is widely used as a spice in India, Pakistan, and China for centuries. It has been suggested that biotransformation of the glycosides by various microorganisms increase the biological activity and bioavailability. This study aims to characterize the biotransformation of the fenugreek saponins by food microorganisms and to assess the cytotoxicity of the biotransformed fenugreek extract and dioscin which is a compound produced during biotrans