김다닐 교수
Danil Kim
서울대학교 · 농업·생명과학
연구실 소개
김다닐 교수의 연구실은 농업 폐기물의 효율적 활용과 동물 건강 및 생산성 향상을 위한 생물학적 기반 기술 개발에 초점을 맞추고 있습니다. 특히 옥수수대를 이용한 리그닌 분해를 통한 바이오연료 및 생화학물질 생산, 가축의 사료 체계가 메탄 배출에 미치는 영향, 그리고 병원성 대장균과 매개균 등 병원성 미생물의 모니터링과 진단 기반의 생물학적 지표 개발을 핵심 연구 방향으로 삼고 있습니다. 또한, 가축의 골다골증 관련 생화학적 마커와 장내 미생물 군집의 변화를 분석하여 건강 상태를 정량화하는 데에도 기여하고 있습니다.
연구 현황
연구 성과 추이
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주요 논문
15Lignin degradation from corn cob utilizing doped nanoparticles for biofuel and biochemical production.
Few studies have examined the effects of feeding total mixed ration (TMR) versus roughage and concentrate separately (SF) on ruminant methane production. Therefore, this study compared differences in methane production, ruminal characteristics, total tract digestibility of nutrients, and rumen microbiome between the two feeding methods in Holstein steers. A total six Holstein steers of initial bodyweights 540 ± 34 kg were divided into two groups and assigned to a same experimental diet with two
Considerable numbers of STEC non-O157 were isolated from cattle farms, and the virulence and antimicrobial resistance features were different between the STEC O157 and non-O157 strains. STEC from cattle with virulence or antimicrobial resistance genes might represent a threat to public health and therefore, continual surveillance of both STEC O157 and non-O157 would be beneficial for controlling and preventing STEC-related illness.
This study determined the appropriate biochemical assay for measuring plasma tartrate-resistant acid phosphatase isoform 5b (TRAP5b) activity; this information is important to clarify the relationship between plasma TRAP5b and known biochemical bone markers in cattle. When plasma TRAP5b was measured using fluorometric and spectrophotometric methods, hemolysis products in plasma did not affect the former method. In plasma from healthy cattle, there was a good correlation (r=0.66) between the 2 me
We examined fluctuations in plasma tartrate-resistant acid phosphatase isoform 5b (TRAP5b) measured using fluorometry in conjunction with those in calcium (Ca) and other bone metabolic markers from 2 weeks prepartum to 2 weeks postpartum in 7 primiparous and 18 multiparous pregnant cows. The plasma Ca concentration decreased temporarily on the day of calving in multiparous cows only. Plasma TRAP5b peaked on the day of calving in primiparous and multiparous cows and was significantly lower in mul
Although Mycobacterium avium subsp. <i>paratuberculosis</i> (MAP) has threatened public health and the livestock industry, the current diagnostic tools (e.g., fecal PCR and enzyme-linked immunosorbent assay [ELISA]) for MAP infection have some limitations, such as inconsistent results due to intermittent bacterial shedding or low sensitivity during the early stage of infection. Therefore, this study aimed to develop a novel biomarker focusing on elucidating the gut microbial signature of MAP-pos
Our objective was to evaluate the effects of a reservoir-type calcitriol (5 mg/animal) transdermal patch on plasma calcitriol and calcium (Ca) concentrations in dairy cattle. A group of six heifers received three different types of patches: an encapsulated reservoir solution containing calcitriol alone (CAL), calcitriol with dodecylamine (CAL+D), or vehicle (CONT). The patches were applied to the skin of the tail for duration of 2 days at intervals of at least 3 weeks. Cattle that received CAL o
<i>Staphylococcus aureus</i> is one of the major pathogens causing bovine mastitis and foodborne diseases associated with dairy products. To determine the genetic relationships between human and bovine or bovine isolates of <i>S. aureus</i>, various molecular methods have been used. Previously we developed an <i>rpoB</i> sequence typing (RSTing) method for molecular differentiation of <i>S. aureus</i> isolates and identification of <i>RpoB</i>-related antibiotic resistance. In this study, we per
How does vaccination against foot-and-mouth disease (FMD) affect pregnant cows? Vaccination is the most effective method of preventing the spread of FMD, but it is linked to sporadic side effects, such as abortion and premature birth, which result in economic loss. In this study, ruminoreticular temperature and body activity were measured before and after FMD vaccination using a ruminoreticular biocapsule sensor in Hanwoo cows at different stages of pregnancy. Compared to the unvaccinated groups
The blood parameters expressing different values between CON and KET in prepartum or early postpartum periods are presumed to show individual nutrition and health states, liver function, and overweight status. These parameters could be valuable indicators that can be used to prevent the occurrence of ketosis and improve management practices by recognizing these differences in ketotic cows before calving.
Holstein calves weighing less than 20 kg at birth have been noted in Korea. Due to insufficient information, we raised small calves with age-matched normal birth weight Holstein calves and determined body weights before puberty. In addition, 3 single nucleotide polymorphisms (SNPs) of the growth hormone (GH) gene were analyzed. Up to 10 months of age, low birth weight calves were smaller than normal weight calves. In exon 5 of the GH gene, SNP genotype variation was detected in some small calves
The aim of the present study was to evaluate the effects of synchronization method, season, parity, corpus luteum (CL) size, and progesterone (P4) levels on the pregnancy rate after bovine embryo transfer (ET). Among 165 recipient candidates who received 1 of 2s estrus synchronization treatments, 96 heifers and 43 cows were selected through rectal examination and used as recipients. The day before ET, the CL size and plasma P4 concentration were evaluated. The CL sizes and plasma P4 levels were
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