김도연 교수

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation i

This paper describes the preparation of 3D polymer monoliths containing internal hierarchical porosity. The porous networks are fabricated based on Pickering high-internal-phase emulsions (HIPEs) stabilized by microporous β-cyclodextrin-based polymer particles (CDPs) as the emulsifier; CDPs are facilely synthesized by the polyaddition reactions without the need for catalysts. The designed Pickering agents enable to form a bicontinuous internal phase in 8:2 cyclohexane-water v/v, and the oil drop

MicroRNAs (miRNAs) are ∼22nt-long single-stranded RNA molecules that form a RNA-induced silencing complex with Argonaute (AGO) protein to post-transcriptionally downregulate their target messenger RNAs (mRNAs). To understand the regulatory mechanisms of miRNA, discovering the underlying functional rules for how miRNAs recognize and repress their target mRNAs is of utmost importance. To determine functional miRNA targeting rules, previous studies extensively utilized various methods including hig

Expansion microscopy (ExM) enhances spatial resolution by using a swellable polymer that expands the sample volume by a factor of ≈4 in one dimension and a factor of ≈64 in volume. Combining ExM with stimulated emission depletion (STED) microscopy, referred to as ExSTED, increases the resolution to up to 10 nm. However, photobleaching is a critical issue in ExSTED because the sample expansion lowers the fluorophore density whereas high-resolution STED requires high depletion intensity. To overco

It has been reported that exogenously introduced micro- RNA (exo-miRNA) competes with endogenously expressed miRNAs (endo-miRNAs) in human cells, resulting in a detectable upregulation of mRNAs with endo-miRNA target sites (TSs). However, the detailed mechanisms of the competition between exo- and endo-miRNAs remain uninvestigated. In this study, using 74 microarrays that monitored the whole-transcriptome response after introducing miRNAs or siRNAs into HeLa cells, we systematically examined the

본 연구의 목적은 인공지능 기술 또는 서비스를 수용하는 사용자의 윤리성 인식을 측정하기 위한 척도 개발 및 타당화에 있다. 이를 위해 인공지능 윤리성 관련 문헌 분석을 통해 구성개념 및 속성을 확인하였다. 전국의 10대-70대 남녀 133명(개방형 설문:1차 문항), 273명(예비조사:2차 문항), 500명(본조사:최종 문항)을 대상으로 실시한 온라인 설문조사 결과를 확인적 요인분석에 의해 정제하여 최종적으로 인공지능기술 윤리성 척도를 개발하였다. 인공지능기술 윤리성 인식 척도는 총 4개 요인(투명성, 안전성, 공정성, 책임성) 16개 문항으로 개발하여 일반적인 인공지능기술 관련 윤리성 인식을 세부 요인별로 측정할 수 있도록 하였다. 개발된 척도를 활용하여 다양한 분야의 측정 변인들과의 관련성을 밝힐 수 있을 것이며, 인공지능기술 발전의 초기 단계에서 윤리성 인식을 높이기 위한 기초 데이터를 제공하는 데 중요한 역할을 할 것으로 기대한다.

We describe the design of functional hierarchical pores in polymer monoliths on the basis of inverse, water-in-oil (w/o), high internal phase emulsions. The emulsion structure was formed and stabilized using functional microporous organic polymers as a Pickering agent, which not only provides an emulsion template to form the multiscale pores but also imparts photocatalytic activity to the polymer monoliths. Thus, inverse Pickering emulsions were obtained from styrene–water (2:8, v/v) with intern

The purpose of this study is to investigate the effect that a Pilates trained group and control group have on the spinal joint range of motion and spinal movement pattern. A group of 8 female pilates instructors with over 1 year experience and a group of 7 females that had no pilates experience participated in this study. Retro-reflective markers were placed on predetermined anatomical locations of participants and dynamic spinal segment motion and radius of curvature was observed using a motion

We investigated the impact of the designed contact area (DCA) and designed contact length (DCL) on material removal rates (MRR) when using a pad with a structured surface in chemical mechanical polishing. The structure of the structured surface pad (SSP) was precisely defined, and an examination was conducted to assess the influence of variations in the shape, size, and spacing of the unit figure (UF) on the MRR. The results revealed that maintaining the DCA constant while altering the UF shape

본 연구는 리더십 개발을 위한 통합적인 요구분석의 방법으로 ‘수행공학을 활용한 접근 방식’을 활용하고, 상황기반적인 요소를 고려하여 ‘우선순위를 결정하는 방안’을 제안하는 것을 연구의 목적으로 하였다. 연구방법으로는 문헌분석과 사례연구를 실시하였다. 수행공학을 활용한 요구분석은 분석영역 선정, 수행분석, 원인분석, 개인요인과 환경요인의 확인의 순으로 이루어졌다. 영업 관리자의 ‘신입 영업인 정착지원’ 영역에 대한 분석 결과, 코칭 스킬 미흡, 사람의 변화가능성에 대한 신념 부족 등의 개인적 요인과 리더역할에 대한 정보제공 부족, 모집인원만을 기준으로 한 평가 및 포상제도 등의 환경적 요인이 수행문제의 원인으로 복합적으로 작용하고 있는 것으로 드러났다. 수행공학을 활용한 요구분석 방식은 거시적이고 통합적인 시각에서 리더십 수행문제의 다양한 원인을 조망할 수 있는 장점을 지니고 있는 것으로 나타났다. 우선순위 결정을 위한 주요 고려 요소로, ‘원인의 중요도’와 ‘개입기법 평가기준’인 타

Bone marrow mesenchymal stem cells (BM-MSCs) play a crucial role in bone formation through their ability to differentiate into osteoblasts. Aging, however, detrimentally affects the differentiation and proliferation capacities of BM-MSCs, consequently impairing bone regeneration. Thus, mitigating the aging effects on BM-MSCs is vital for addressing bone-related pathologies. In this study, we demonstrate that extracellular nanovesicles isolated from gold kiwi (GK-NVs) protect human BM-MSCs from u