권혁준 교수
Hyuk-Joon Kwon
서울대학교 · 의학
연구실 소개
권혁준 교수의 연구실은 인플루엔자 바이러스의 유전자 기반 변형과 병원성 조절을 핵심으로 하며, 특히 가금류에 기생하는 아형 인플루엔자 A 바이러스 및 병원성 대장균의 항생제 내성 메커니즘을 분석하고 있습니다. 또한 라이소좀 저장질환인 GM1 갈락토시드증의 뇌영상학적 특성과 병태생리학적 진행을 규명하는 데에도 기여하고 있습니다. 연구는 바이러스의 숙주 적응 메커니즘과 병원성 조절, 항생제 내성 유전자 전파를 중심으로 진행되고 있습니다.
연구 현황
연구 성과 추이
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주요 논문
15To examine the genetic background of avian pathogenic Escherichia coli (APEC) that affects virulence of this microorganism, we characterized the virulence genes of 101 APEC strains isolated from infected chickens between 1985 ˜ 2005. Serotypes were determined with available anti-sera and median lethal doses were determined in subcutaneously inoculated chicks. The virulence genes we tested included ones encoding type 1 fimbriae (fimC), iron uptake-related (iroN, irp2, iucD, and fyuA), toxins (lt,
In this article, we describe the characteristics of 12 human colorectal-carcinoma cell lines established from 6 primary tumors and 6 metastatic sites of 11 Korean colorectal-carcinoma patients, including the morphology in vivo and in vitro and mutations of K-ras2, p15, p16, p53, APC, beta-catenin, hMLH1 and hMSH2 genes in vitro. No lines were contaminated with Mycoplasma or bacteria. All lines were proven to be unique by DNA-fingerprinting analysis. All lines expressed the surface carcino-embryo
This study to identify anti-influenza components of the bark of Alnus japonica resulted in the isolation of four lupane-type triterpenes (1-4) and one steroid (5). Their structures were characterized on the basis of physicochemical properties, NMR evidence, and ESI-MS data compared with reported data in the literature. Betulinic aldehyde (3) exhibited a particularly strong anti-influenza effect against KBNP-0028 relative to a positive control.
The nucleotide sequences of a region including S1, S2, 3a, 3b and E genes of twenty-seven infectious bronchitis virus (IBV) isolates in Korea between 1990-2011 were determined and phylogenetic and computational recombination analyses were conducted. The sizes of coding regions of some genes varied among IBV isolates due to deletion or insertion of nucleotides; the nucleotide similarities of S1, S2, 3a, 3b and E genes among the 27 isolates were 75.9%-100.0%, 85%-100.0%, 64.0%-100.0%, 60.4%-100.0%
Serotyping has been the gold standard for identifying Salmonella, but it requires large amounts of standard antisera. Multilocus sequence typing (MLST) has been applied to identify Salmonella serovars, but the recombination of 4–7 housekeeping genes and multiple analytic steps diminish its applicability. In the present study, we determined the complete sequences of the RNA polymerase beta subunit gene (rpoB) and 7 housekeeping genes (aroC, dnaN, hemD, hisD, purE, sucA, and thrA) for 76 strains o
In dieser Arbeit wird versucht, Sönke Wortmanns erfolgreichen Fußballfilm Das Wunder von Bern (2003) aus der Perspektive der kollektiven Identität zu analysieren. In dem Film wird die Geschichte von Deutschlands unerwartetem Sieg bei der Fußball-Weltmeisterschaft 1954 in Bern erzählt. Das als ‘Wunder von Bern’ genannte Ereignis wird oft als ‘echte Geburtsstunde’ Deutschlands gewertet,weil die Deutschen durch diesen Sieg zum ersten Mal nach dem Krieg wieder zu großem Selbstbewusstsein gelangten.
Rapid and accurate detection of H5Nx avian influenza viruses is critical for effective surveillance and control measures. Currently, RT-qPCR with spin column RNA extraction is the gold standard for HPAIV surveillance, but its long reaction time and need for specialized equipment limit its effectiveness for rapid response. In this study, we introduce a centrifuge-free, rapid detection method for on-site detection of H5Nx viruses that combines magnetic bead-based ribonucleoprotein (RNP) purificati
Der biblische Sündenfallmythos besitzt sowohl in Kafkas Denken als auch in seinem Werk einen hohen Stellenwert. In dieser Arbeit habe ich versucht darzustellen, wie Kafka in seiner Erzählung Das Urteil die Gestaltung dieses Mythos unternimmt. Für die Annahme, dass Kafkas Gedankenwelt von dem biblischen Sündenfallmythos stark geprägt ist, spricht zunächst seine intensive Beschäftigung mit diesem Mythos, vor allem aus den Jahren 1917 und 1918. Wichtige Punke in Kafkas Exegese des Sündenfallmythos
In dieser Arbeit wird versucht, das Inzestmotiv in Wagners Ring des Nibelungen (1851-1874) und in dem koreanischen Film Oldboy (2003) vergleichend zu untersuchen, um eine Art und Weise der Darstellung des sozialen Tabus in der modernen Kunst festzustellen. Der Inzest ist eines des wenigen universellen Tabus der Menschheit. In der deutschen Literatur wird der vollzogene Inzest zwischen Verwandten meist hart bestraft. Dabei geht es um gesellschaftliche Praxis und nicht um naturrechtliche Prinzipie
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram
In dieser Arbeit wird versucht, die Sprachkritik um die Jahrhundertwende anhand der Texte von Nietzsche und Mauthner zu analysieren, um ihre Bedeutung für die literarische Moderne auszuloten. Bei der Darstellung der zentralen Kritikpunkte Nietzsches sind zwischen zwei Papadigmata, Wortebene und syntaktischer Ebene, zu unterscheiden. Auf der Wortebene zeigt sich, dass es sich bei Wörtern ursprünglich um Metaphern handelt, also um Übertragung von unvollkommenen, auf Ähnlichkeiten beruhenden Abbild
In der vorliegenden Arbeit wird versucht, Kafkas Umgang mit dem grundlegenden Wandel technischer Speichermedien um 1900, die mit einer Entprivilegierung von Schriftsprachlichkeit einhergeht, anhand seiner Texte zu beleuchten. Die Veränderung jener Zeit betreffen nicht nur Kulturproduktion,sondern auch die ganze Lebens- und Erfahrungswelt der Menschen. Mechanisierung und Bürokratisierung der Gesellschaft stehen im Zeichen neuer technischer Übertragungsmöglichkeiten. Die Lebenszeit Kafkas und somi
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