박진봉 교수

Making up for lost time: The one-pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z535

Converging on the goal: New chemistry enables the key Cys-peptide-(αthioester) intermediate to be extended by ligation at either the N or C termini in a controlled fashion. This approach forms the basis for a set of novel tactics for the fully convergent synthesis of proteins by the chemical ligation of multiple peptide segments. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600702_s.pdf or from the author. Please note: The p

Striking similarity: An efficient synthetic route to native ubiquitin and its diastereomer [D-Gln 35]ubiquitin was realized by combining a one-pot native chemical ligation process with protein desulfurization. High-resolution X-ray crystallographic studies of the protein diastereomer (see picture) revealed a striking conservation of molecular structure. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z463040_s.pdf or from the au

Zeitsparend: Die Eintopfsynthese von Crambin (siehe Struktur) liefert nach nur zwei Tagen und einem einzigen abschließenden Reinigungsschritt ca. 40 % Gesamtausbeute an hochreinem Protein. Durch native chemische Ligation werden dabei drei nichtgeschützte Peptidsegmente verknüpft. Die Polypeptidkette faltet sich auch ohne Zwischenreinigung zur 3D-Struktur.

Crambin is a small (46 amino acids) protein isolated from the seeds of the plant Crambe abyssinica. Crambin has been extensively used as a model protein for the development of advanced crystallography and NMR techniques and for computational folding studies. We set out to establish synthetic access to crambin. Initially, we synthesized the 46 amino acid polypeptide by native chemical ligation of two distinct sets of peptide segments (15 + 31 and 31 + 15 residues). The synthetic polypeptide chain

Dem Ziel nahe: Mit einer neuen Strategie gelingt es, das Schlüsselintermediat Cys-Peptid-αThioester durch Ligation am N- oder am C-Terminus kontrolliert zu verlängern. Dieser Ansatz ist die Grundlage für eine vollständig konvergente Synthese von Proteinen durch die chemische Ligation einer Vielzahl von Peptidsegmenten. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2006/z600702_s.pdf or from the author. Please note: The publisher is

DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells. In this review, we introduce single-cell DNA-methylation profiling methods, including experimental strategies and approaches to computational data analysis. Furthermore, the blind spots of the basic analysis and recent alternatives are briefly

To make more practical the total chemical synthesis of proteins by the ligation of unprotected peptide building blocks, we have developed a method to facilitate the isolation and handling of intermediate products. The synthetic technique makes use of a His6 tag at the C terminus of the target polypeptide chain, introduced during the synthesis of the C-terminal peptide segment building block. The presence of a His6 tag enables the isolation of peptide or protein products directly from ligation re

[reaction: see text] A peptide-(alpha)thiophenylester is a key reactant in native chemical ligation. Preformation of the peptide-(alpha)thiophenylester could be useful for enhancing the ligation reaction. We report the direct on-resin preparation of preformed peptide-(alpha)thiophenylesters using a simple and efficient method. The peptide-(alpha)thiophenylester reacted extremely rapidly with a Cys-peptide when compared to the peptide-(alpha)thioalkylester.

Verblüffende Ähnlichkeit: Eine effiziente Synthese von nativem Ubiquitin und seinem Diastereomer [D-Gln35]ubiquitin gelang durch die Kombination einer chemischen Ligation als Eintopfverfahren mit Proteinentschwefelung. Hochauflösende röntgenkristallographische Studien am Proteindiastereomer (siehe Bild) enthüllten verblüffend ähnliche Molekülstrukturen.

Chronic lymphocytic leukemia (CLL) is extremely rare in Asian countries and there has been one report on genetic changes for 5 genes (TP53, SF3B1, NOTCH1, MYD88, and BIRC3) by Sanger sequencing in Chinese CLL. Yet studies of CLL in Asian countries using Next generation sequencing have not been reported. We aimed to characterize the genomic profiles of Korean CLL and to find out ethnic differences in somatic mutations with prognostic implications. We performed targeted sequencing for 87 gene pane

UTX is a histone demethylase gene located on the X chromosome and is a frequently mutated gene in urothelial bladder cancer (UBC). UTY is a paralog of UTX located on the Y chromosome. We performed target capture sequencing on 128 genes in 40 non-metastatic UBC patients. UTX was the most frequently mutated gene (30%, 12/40). Of the genetic alterations identified, 75% were truncating mutations. UTY copy number loss was detected in 8 male patients (22.8%, 8/35). Of the 9 male patients with UTX muta

Existing methods to enrich target regions of genomic DNA based on PCR, hybridization capture, or molecular inversion probes have various drawbacks, including long experiment times and low throughput and/or enrichment quality. We developed CRISPR-Cap, a simple and scalable CRISPR-based method to enrich target regions of dsDNA, requiring only two short experimental procedures that can be completed within two hours. We used CRISPR-Cap to enrich 10 target genes 355.7-fold on average from Escherichia