김경미 교수

Cells release a range of membrane-enclosed extracellular vesicles (EVs) into the environment. Among them, exosomes and microvesicles (collectively measuring 40-1000 nm in diameter) carry proteins, signaling lipids, and nucleic acids from donor cells to recipient cells, and thus have been proposed to serve as intercellular mediators of communication. EVs transport cellular materials in many physiologic processes, including differentiation, stem cell homeostasis, immune responses, and neuronal sig

Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts to eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, the differential presence of DPP4 allowed flow cytometry-mediated isolation of senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cy

This article is the first outcome of a two-part study examining American college students' perceptions of South Korea as a travel destination. The study, as a whole, verifies whether students' destination image formation is affected differently by their travel experience in a destination. In other words, this article investigates whether students' travel experience during college reinforces their image formation in a destination and affects their future travel decision making and choices. A tota

This study investigates how tourism affects the quality of life of residents in tourism destinations that vary in the stage of development. The proposed model in this study structurally depicts that satisfaction with life in general derives from the satisfaction with particular life domains. Overall life satisfaction is derived from material well-being, which includes the consumer's sense of well being as it is related to material possessions, community well-being, emotional well-being, and heal

During or right after mRNA export via the nuclear pore complex (NPC) in mammalian cells, mRNAs undergo translation mediated by nuclear cap-binding proteins 80 and 20 (CBP80/20). After CBP80/20-dependent translation, CBP80/20 is replaced by cytoplasmic cap-binding protein eIF4E, which directs steady-state translation. Nonsense-mediated mRNA decay (NMD), one of the best-characterized mRNA surveillance mechanisms, has been shown to occur on CBP80/20-bound mRNAs. However, despite the tight link betw

Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA2 inhibition by annexin-I was consistent with a 'specific int

A microarray-chip assay system for the fluorescence detection of phosphate-containing analytes in aqueous media has been constructed from stimuli-responsive polymerized poly(diacetylene)-liposomes for the first time. Proper combination of the liposome components (Zn(II)-dipicolylamine for phosphate binding and an amine-terminated component for anchoring the liposome onto an aldehyde-derivatized glass plate), has led to a microarray chip that selectively detects pyrophosphate, an important biomar

Alzheimer's disease (AD) is the most common type of dementia. Amyloid β (Aβ) plaques, tau-containing neurofibrillary tangles, and neuronal loss leading to brain atrophy are pathologic hallmarks of AD. Given the importance of early diagnosis, extensive efforts have been undertaken to identify diagnostic and prognostic biomarkers for AD. Circulating extracellular vesicles (EVs) provide a platform for "liquid biopsy" biomarkers for AD. Here, we characterized the RNA contents of plasma EVs of age-ma

Mitochondria are cytosolic organelles essential for generating energy and maintaining cell homeostasis. Despite their critical function, the handful of proteins expressed by the mitochondrial genome is insufficient to maintain mitochondrial structure or activity. Accordingly, mitochondrial metabolism is fully dependent on factors encoded by the nuclear DNA, including many proteins synthesized in the cytosol and imported into mitochondria via established mechanisms. However, there is growing evid

삶의 의미는 노년기의 행복과 건강에 중요한 역할을 한다. 그러나 기존 연구들은 삶의 의미가 미치는 영향력을 광범위하게 살펴보지 못하였고, 연령의 증가에 따른 삶의 의미의 중요성도 간과하였다. 이에 본 연구에서는 노년기에 경험하는 삶의 의미가 행복과 건강의 구체적인 영역에 미치는 영향력이 청년기와 비교하여 얼마나 강한지 알아보고자 하였다. 연구에 참여한 사람들은 총 409명으로 서울지역에 거주하는 20대 대학생 203명(평균연령: 22.10세)과 60대 이상의 206명(평균연령: 67.40세)이었으며, 설문 내용은 삶의 의미, 행복, 심리적․신체적 건강관련 불편증상, 수면 및 건강관련 행동(규칙적인 식사와 운동, 음주횟수, 흡연량) 등으로 구성되었다. 분석 결과 20대와 60대 이상 모두에게서 삶의 의미는 행복과 정적상관이 있었으나, 그 영향력 정도는 20대보다 60대 이상에서 더 강한 것으로 나타났다. 또한 20대에 비해 60대 이상에서 삶의 의미의 영향력이 행복을 포함하여, 수면의

ABSTRACT Festivals and special events serve as important attractors for destinations and provide unique experiences for visitors. These events also have the capability of providing both tangible and intangible benefits for communities. Most of the studies dealing with festivals and events have focused on either understanding attendees' behavior or delineating the economic impacts of visitation upon communities in which such events are held. However, little research has been done to examine how d

The senescence-associated secretory phenotype (SASP) is a major trait of senescent cells, but the molecular regulators of SASP factor secretion are poorly understood. Mass spectrometry analysis revealed that secretory carrier membrane protein 4 (SCAMP4) levels were strikingly elevated on the surface of senescent cells compared with proliferating cells. Interestingly, silencing SCAMP4 in senescent fibroblasts reduced the secretion of SASP factors, including interleukin 6 (IL6), IL8, growth differ