京都大学 · 生化学・遺伝学・分子生物学
Yuki Takahashi教授の研究室は、細胞間コミュニケーションを担う小胞体（sEV）の生物学的機能と動態を解明することを目的としています。特に、血液中におけるsEVの産生と消失のキネティクスを定量的に解析する新規技術の開発に注力しており、がんや疾患におけるsEVの役割を解明する基盤を構築しています。また、膜タンパク質の機能やリン脂質修飾がsEVの体内動態に与える影響についても、標識技術とバイオインフォマティクスを融合した革新的なアプローチを展開しています。
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Small extracellular vesicles (sEVs) are important mediators of cell-cell communication with respect to diverse physiological processes. To further understand their physiological roles, understanding blood sEV homoeostasis in a quantitative manner is desired. In this study, we propose novel kinetic approaches to estimate the secretion and clearance of mouse plasma-derived sEVs (MP-sEVs) based on the hypothesis that blood sEV concentrations are determined by a balance between the secretion and cle
Exosomes are extracellular vesicles released by various cell types and play roles in cell-cell communication. Several studies indicate that cancer cell-derived exosomes play important pathophysiological roles in tumor progression. Biodistribution of cancer cell-derived exosomes in tumor tissue is an important factor for determining their role in tumor proliferation; however, limited studies have assessed the biodistribution of exosomes in tumor tissues. In the present study, we examined the effe
Extracellular vesicles (EVs) are small membrane vesicles secreted from cells and have great potential as drug delivery carriers. Surface proteins on EV membranes might play roles in pharmacokinetics. One method which can be used to study the role of surface membrane of EV is to modify the inner space of EV. In the present study, we constructed a plasmid DNA expressing a fusion protein of Gag protein derived from Moloney murine leukemia virus (Gag) and Gaussia luciferase (gLuc) (Gag-gLuc) to modi
Small extracellular vesicles (sEVs) are important mediators of intercellular communication with respect to diverse pathophysiological processes. Here, we determined novel phosphatidylserine (PS)-deficient sEV subpopulations as a major somatic cell-derived sEV subpopulation in blood because of long blood circulation half-life through escape from macrophage uptake. PS<sup>(-)</sup>-sEVs were identified in various cultured cells as a minor population. However, as a result of rapid uptake of PS<sup>
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