Donghyun Lim

MicroRNAs (miRNAs) regulate gene expression by targeting protein-coding transcripts that are involved in various cellular processes. Thus, miRNA biogenesis has been recognized as a novel therapeutic target. Especially, the let-7 miRNA family is well-known for its tumor suppressor functions and is downregulated in many cancer cells. Lin28 protein binds to let-7 miRNA precursors to inhibit their maturation. Herein, we developed a FRET-based, high-throughput screening system to identify small-molec

Genetically fusing protein domains to Cas9 has yielded several transformative technologies; however, the genetic modifications are limited to natural polypeptide chains at the Cas9 termini, which excludes a diverse array of molecules useful for gene editing. Here, we report chemical modifications that allow site-specific and multiple-site conjugation of a wide assortment of molecules on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse functions. Usi

Abnormal function of RNA-binding proteins can lead to dysregulation of RNA function, causing a variety of disease states. Thus, developing small-molecule modulators of protein-RNA interactions is one of the key challenges in chemical biology. Herein, we performed a high-throughput screening of chemical libraries using a Förster resonance energy transfer-based Lin28-let-7 interaction assay to identify a potent small-molecule inhibitor of the protein-microRNA interaction, as it is an important tar

Even though persistent neural activity has been proposed as a mechanism for maintaining eligibility trace, direct empirical evidence for active maintenance of eligibility trace has been lacking. We recorded neuronal activity in the medial prefrontal cortex (mPFC) in rats performing a dynamic foraging task in which a choice must be remembered until its outcome on the timescale of seconds for correct credit assignment. We found that mPFC neurons maintain significant choice signals during the time

Herein, we report the synthesis of fused-triazole scaffolds that are connected by pyrimidines, pyrazoles, or pyrazolopyrimidines through carbohydrate-derived stereodivergent linkers. Pyrimidine-, pyrazole-, or pyrazolopyrimidine-based carbohybrids were constructed through condensations of the key intermediates, 2-C-formyl glycals, with various dinucleophiles. Fused-triazole scaffolds were obtained through intramolecular 1,3-dipolar cycloadditions after selective functionalization of the carbohyb

Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome-editing technologies have revolutionized biology, biotechnology, and medicine, and have spurred the development of new therapeutic modalities. However, there remain several barriers to the safe use of CRISPR technologies, such as unintended off-target DNA cleavages. Small molecules are important resources to solve these problems, given their facile delivery and fast action to enable temporal control of the CRISPR sys

In the BEOL (back end of line) process, tungsten (W) chemical mechanical planarization (CMP) has been commonly used to form contacts. As size of the metal line is shrunk, metal CMP will directly impacts resistance and reliability characteristic of final product. As examples, W contact recess and seam (or void) defects make resistance problems and block failure. In the W CMP Process, W is oxidized by etchant, and then removed by abrasives. Progressively, this process is cycled again until all bul

본 연구의 목적은 공개공지를 구성하는 물리적 측면의 요소들이 이용자들에게 어떤 영향을 미치고 있는지를 실증분석 하는 것이다. 이를 위해, 독립변수는 공개공지의 물리적 특성, 공개공지와 가로와의 관계, 공개공지의 입지유형, 건물 저층부 시설유형으로 분류하고, 종속변수는 공개공지 총이용인원, 건물내부이용인원, 외부이용인원, 선택적 활동인원, 사회적 활동인원으로 구분된 5가지의 영향관계 모형을 설정한다. 연구의 결과, 공개공지의 면적과 건물의 측면과 코너부에위치한 공개공지는 모형의 유형과 관계없이 공통적 영향요인으로 나타났다. 반면, 횡단보도 입지형과 개방형 공개공지는 외부이용인원 모형과 사회적 활동인원 모형에 영향을 미치며, 지하철 입지형 공개공지는 건물내부이용인원 모형과 선택적 활동인원 모형에 영향을 미치는 것으로 나타났다. 위의 결과를 바탕으로 공개공지의 면적을 증가시키는 방안, 주변의 시선을 차폐시키는 방안,접근성 향상을 위한 평면적 형태로의 조성방안을 제안한다. 이러한 제안에 대

We report the controlled dielectrophoretic assembly for the deterministic fabrication of carbon nanotube (CNT) probes. Electrical detection was applied to the dielectrophoretic assembly of CNT probes. Dielectrophoretic manipulation with an ac electric field of 5 MHz was used to form the CNT bridge across oppositely aligned tungsten tips (W-tips). A dc electric field was simultaneously applied to monitor the direct current flowing through the gap. The detected nanocurrent reveals that the CNT bri

This letter presents a method for active noise cancelation (ANC) for headphone application. The method improves the performance of ANC by deriving a flexible independent component analysis (ICA) algorithm in a hybrid structure combining feedforward and feedback configurations with correlation-based wind detection. The effectiveness of the method is demonstrated through simulation.