Doo-San Baek

Effective therapies are urgently needed for COVID-19. Here we describe the identification of a new stable human immunoglobulin G1 heavy-chain variable (VH) domain scaffold that was used for the construction of a large library, lCAT6, of engineered human VHs. This library was panned against the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) glycoprotein. Two VH domains (VH ab6 and VH m397) were selected and fused to Fc for increased half-life in circulation. The VH-Fc ab6 and m397 spec

Procollagen type I carboxy-terminal propeptide (PICP), derived from type I procollagen, has been identified as an indicator of type I collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting of two α1 chains (PICPα1) and one α2 chain (PICPα2). Here, we report the recombinant expression of human PICP using a mammalian expression system. Co-expression of PICPα1 and PICPα2 in HEK293F cells resulted in the production of functional PICP in the co

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed l

Metastasis and chemoresistance in colorectal cancer are mediated by certain poorly differentiated cancer cells, known as cancer stem cells, that are maintained by Notch downstream signaling initiated upon Notch cleavage by the metalloprotease ADAM10. It has been shown that ADAM10 overexpression correlates with aberrant signaling from Notch, erbBs, and other receptors, as well as a more aggressive metastatic phenotype, in a range of cancers including colon, gastric, prostate, breast, ovarian, ute

Neutrophil elastase (NE) is a serine protease released during neutrophil maturation. High levels of NE are related to lung tissue damage and poor prognosis in cancer; thus, NE is a potential target for therapeutic immunotherapy for multiple lung diseases and cancers. Here, we isolate and characterize two high-affinity, specific, and noncompetitive anti-NE antibodies Fab 1C10 and VH 1D1.43 from two large phage-displayed human Fab and VH libraries. After fusion with human IgG1 Fc, both of them (VH

Chimeric antigen receptor-T (CAR-T) cells and antibody-drug conjugates (ADCs) are promising therapeutic strategies in oncology. The carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is overexpressed in tumors including non-small cell lung cancer (NSCLC) and pancreatic ductal adenocarcinoma (PDAC), and is an attractive target for therapies based on CAR-T cell or/and ADCs. We previously developed a highly specific antibody-based CAR-T cells targeting CEACAM5 and the tumoricidal e

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) is a type II transmembrane glycoprotein expressed in many tissues. High expression levels of ENPP1 have been observed in many cancer types such as lung cancer, ovarian cancer, and breast cancer. Such overexpression has been associated with poor prognosis in these diseases. Hence, ENPP1 is a potential target for immunotherapy across multiple cancers. Here, we isolated and characterized two high-affinity and specific anti-ENPP1 Fab antibod

Neuropilin-1 (NRP1), which functions as a coreceptor for vascular endothelial growth factor (VEGF) and is implicated in vascular permeability and tumorigenesis, has been targeted by peptides that specifically bind to the VEGF-binding region on NRP1. Like natural VEGF ligands, all known peptides with NRP1-binding activity bind only through a carboxy (C)-terminal R/K-x-x-R/K sequence motif (x stands for any amino acids); this strict requirement is called the C-end rule (CendR). Here, we report imm

Because of the properties of software such as invisibility, complexity, and changeability, software configuration management (SCM) for software artifacts generated during software life-cycle has been used for guarantee of the quality of the software. However, the existing SCM system has only focused on code artifacts and software development document artifacts such as Software Requirements Specification (SRS), Software Design Description (SDD), and Software Test Description (STD). Moreover, soft

국문초록이 논문은 새로 발굴된 원우전(元雨田)(1895-1970)의 무대디자인과 생애자료를 중심으로 무대미술 활동의 의미를 규명하는 시론(試論)이다. 원우전의 본명은 원세하(元世夏)로, 서울에서 태어나 경신학교, 서화미술회에서 수학하고 <백조> 동인으로 활동하였다. 이 시절 홍사용 등의 회고에서 엿보이는 조선적인 것에 대한 관심과 낭만적 기질은 그의 예술을 관통하는 화두이다. 서양화가들이 주도하였던 토월회 1기 공연의 성과와 한계를 바탕삼아 원우전은 김운선, 미다라이(御手洗) 등의 무대장치가와 교류, 무대기술을 연구하였고, ‘전이모사’와 세밀화 기술 등 동양화 제작 방식을 무대미술 제작에 응용하였다. 2기 토월회 미술을 주도한 원우전은 입체무대와 배경막의 절충으로 당대 연극무대의 한계였던 일본 가부키식의 전형적 무대미술을 혁신했다. 1929년 토월회 <아리랑 고개> 무대의 핵심인 ‘길’은 배경막과 입체무대를 절충하여 긴 거리감을 조성하여 민족의 이산(離散)을 효과적으로 표현하는 ‘조

이 연구는 임희재의 희곡 <꽃잎을 먹고 사는 기관차>에 나타난 전쟁미망인의 형상화와 그 의미를 살피고, 전후희곡에 등장하는 전쟁미망인과 상이군인 형상화의 특이성을 규명하려는 시론(試論)이다. 이 작품은 전쟁미망인을 다룬 여타의 희곡과 달리 ‘실종된 남편이 살아 돌아온다면’이라는 가정을 서스펜스의 축으로 삼는다. 이 과정에서 전쟁미망인을 둘러싼 사회적 담론과 극텍스트가 구현하는 영자의 서사는 극 안에서 분열하는 양상을 보인다. 50년대 전쟁미망인에 대한 사회적 담론은 극텍스트에서 신문광고와 박형래의 유서(遺書)를 통해 제시된다. <꽃잎을 먹고 사는 기관차>의 특이성은 바로 이러한 공적 영역의 담론들에 대하여 전쟁미망인 영자가 대응하고 있다는 것이다. 극텍스트에서 김영자는 질문에 대하여 답변하며, ‘증언’의 방식으로 과거를 진술한다. 증언을 통한 개인의 과거와 신문광고, 유서 텍스트의 정보는 서로 경쟁관계에 놓인다. 나아가 김영자는 옷차림의 변신, 육체의 발견과 패션의 모방, 재가의 욕