Geum-hee Yun

High levels of mitotic progression-associated PLK1 and stress-associated HSF1 have been observed in various human cancers. In the present study, we investigated the effects of PLK1 and HSF1 knockdown on the proliferation of oral cancer cells using small interfering RNA. In human oral squamous cell carcinoma (SCC) tissues, the levels of PLK1 and HSF1 were higher compared to normal tissues. The expression levels of PLK1 and HSF1 were also elevated in the human oral SCC cell lines FaDu and HEp-2. D

The mutated K-ras gene is involved in approximately 30% of human cancers. In order to search for K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice, we performed microarray and proteomics (LC/ESI-MS/MS) analysis. Genes (RAB27b RAS family, IL-1RA, IL-33, chemokine ligand 6, epiregulin, EGF-like domain and cathepsin) related to cancer development (Wnt signaling pathway) and inflammation (chemokine/cytokine signaling pathway, Toll receptor signaling) were up-regulated while

Nasal-type T/natural killer (NK) cell lymphoma, which often shows an angiocentric growth pattern, is a distinct clinicopathological entity highly associated with the Epstein-Barr virus (EBV). This tumour has a characteristic immunophenotype, whereas the cytological spectrum is broad. It is known that a clonal T-cell receptor (TCR) gene rearrangement is not found in this tumour. However, it is still unresolved as to whether the finding of a clonal TCR gene rearrangement excludes the diagnosis of

It has been reported that the SW480 human colon cancer cell line consists of E-type and R-type cells. The long-term tumorigenic potential, invasive and metastatic properties of these subclones have not been characterized. E-type and R-type cells were subcloned using limiting dilution methods from parental SW480 cells. The cell growth rate was determined by MTT colorimetric assay, and colony forming efficiency was analyzed using Matrigel-coated plates. The activity of matrix metalloproteinase (MM

瞄准：在高肥的导致食谱的肥胖的老鼠的下的脂肪组织在类脂化合物新陈代谢和 FABP mRNA 表示上调查 Platycodon grandi-florum 摘录(PGE ) 的效果。方法：PGE 被对待在 pre-adipocyte 3T3-L1 区别和胰脂肪活动上调查禁止的效果。有 439.03 +/- 的平均重量的男 Sprague-Dawley 老鼠 7.61 g 被划分成四个组：独自喂了一本试验性的食谱的控制组(C 和 H 组) 和在 150 mg/kg 体重的集中与一本控制食谱或 HFD 一起管理了 PGE 的 PGE 治疗组(C + PGE 和 H + PGE 组，分别地) 为 7 wk。血浆全部的胆固醇(TC ) 和 triglycerol (TG ) 集中从老鼠的尾巴静脉被测量。Adipocyte 房间区域从下的脂肪组织被测量，脂肪酸绑定蛋白质(FABP ) mRNA 表示被北污点分析分析。结果：PGE 处理禁止了 3T3-L1 pre-adipocyte 区别和胖累积，并且也减少了胰脂肪活动。在这个实验， PGE 显著地减少了血浆 TC 和 TG 集中以及体重和下的脂肪

Aromatase encoding by the CYP19 gene catalyzes the conversion of androgens to estrogens. In order to determine if polymorphisms of the CYP19 gene are associated with breast cancer risk, we analyzed the frequency of tetranucleotide (TTTA) tandem repeats and a 3-bp insertion (I)/deletion (D) polymorphism in intron 4 of the CYP19 gene in genomic DNA from 70 Korean breast cancer patients and 102 age-matched, healthy women. The 3-bp deletion allele was found more frequently in the breast cancer group

Odontoblasts are involved in tooth repair and regeneration as well as dentin formation. The aim of this study was to examine whether delivery of heat shock protein 27 (Hsp27) into cells using a TAT fusion protein system (TAT-Hsp27) enhances adhesion and migration of murine dental papilla-derived MDPC-23 cells. Hsp27 was delivered into cells by the TAT-fusion protein system. To examine whether TAT-Hsp27 affects the viability of MDPC-23 cells, MTT assay was performed. The effect of TAT-Hsp27 on ad

본 연구는 광동댐 유역을 대상으로 RCPs (Representative Concentration Pathways) 기후변화 시나리오의 Arc-SWAT 적용으로 평균유출량과 저유량 계열을 구축하고 경계핵함수(Boundary Kernel)를 이용하여 비매개변수적 갈수빈도 해석을 수행하였다. 분석결과, RCPs 시나리오 하에서 가까운 미래의 유출량 감소로 인한 가뭄발생빈도가 증가하였으며, RCP8.5에서 저유량 계열의 변동폭이 크게 나타났다. Median flow의 갈수량 빈도해석결과 가까운 미래(2030s)의 30년 빈도 갈수량의 경우 Historic 기간에 비하여 증가(RCP4.5: +22.4%, RCP8.5: +40.4%)하였으나, 먼 미래(2080s)에는 갈수량 감소(RCP4.5: -4.7%, RCP8.5: -52.9%)로 인한 가뭄발생빈도가 커지는 것으로 분석되었다. 또한 Quantile 25% flow 저유량 계열의 경우 먼 미래에 빈도별 갈수량이 감소(RCP4.5: -20.8

The E6 and E7 oncoproteins of human papilloma virus (HPV) type 16 have been known to cooperatively induce the immortalization and transformation of primary keratinocytes. We established an E7 transgenic mouse model to screen HPV-related biomakers using the omics approach. The methods used to identify HPV-modulated factors were genomics analysis by microarray using the Affymetrix 430 2.0 array to screen E7-modulated genes, and proteomics analysis using nano-LC-ESI-MS/MS to screen E7-modulated pro

In previous research, we focused on the discovery of K-ras biomarkers, and effects of genotoxic carcinogens on their expression were investigated in this study. It is well-known that mutated K-ras gene is involved in approximately 30% of human cancers such as lung cancer. To search for K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice, we analyzed K-ras-specific genes and proteins related to cancer development, signal transduction, inflammation as well as tumor suppressi

A phase-locked (PLL) with a charge pump boosting technique is described. The technique enables the voltage controlled oscillator circuit in the PLL to run faster than conventional circuits at low supply voltage. This design method is applicable to PLLs with low jitter, high-speed characteristics in environments with high supply noise.