Gyun Min Lee
Korea Advanced Institute of Science and Technology · 生化学・遺伝学・分子生物学
研究室紹介
Professor Gyun Min Lee's research lab specializes in bioprocess engineering and mammalian cell culture optimization for the efficient production of therapeutic proteins, particularly monoclonal antibodies and recombinant erythropoietin. The lab investigates key culture parameters such as pH, temperature, osmolality, and chemical additives like sodium butyrate to enhance cell viability, productivity, and product titer in serum-free suspension cultures of CHO and hybridoma cells. A major focus is on developing novel biologics—such as the double-antiangiogenic protein (DAAP)—for treating inflammatory diseases like rheumatoid arthritis, often in combination with existing biologics like TNF-α inhibitors. The lab also explores molecular tools, including the WPRE element, to improve transient gene expression systems in HEK293E cells for rapid, high-yield protein production.
Research Overview
Research Output Trend
Figures are computed from collected data and may differ slightly.
Selected Papers
15To investigate the effect of culture pH in the range of 6.85-7.80 on cell growth and erythropoietin (EPO) production at 32.5 and 37.0 degrees C, serum-free suspension cultures of recombinant CHO cells (rCHO) were performed in a bioreactor with pH control. Lowering culture temperature from 37.0 to 32.5 degrees C suppressed cell growth, but cell viability remained high for a longer culture period. Regardless of culture temperature, the highest specific growth rate (mu) and maximum viable cell conc
Cell culture longevity in fed-batch culture of hybridomas is often limited by elevated medium osmolality caused by repeated nutrient feeding. Shotwise feeding of 10x Dulbecco's modified Eagle's medium (DMEM) concentrates elevated the osmolality of medium up to 540 mOsm/kg at the end of fed-batch culture of S3H5/gamma2bA2 hybridoma which is known to be lethal to most hybridomas. S3H5/gamma2bA2 hybridoma has been shown to grow without significant growth depression at 219 mOsm/kg in DMEM supplement
Chinese hamster ovary (rCHO) clones in response to culturepH and temperature, serum-free suspension cultures of twoantibody-producing CHO clones (clones A and B), which wereisolated from the same parental clone by the limiting dilutionmethod, were performed in a bioreactor at pH values in therange of 6.8-7.6, and two diferent temperatures, 33oC and37oC. In regard to cell growth, clone A and clone B displayedresponse difered. In contrast, clones A and B displayed diferentresponses to temperature
INTRODUCTION: Angiogenesis plays a critical role in synovial inflammation and joint destruction in rheumatoid arthritis (RA). Vascular endothelial growth factor A (VEGF-A) and angiopoietins are two important mediators of synovial angiogenesis. We have previously developed a novel chimeric decoy receptor, namely, double-antiangiogenic protein (DAAP), which can both bind VEGF-A and angiopoietins and block their actions. This study was performed to evaluate the antiarthritic effect of DAAP and the
Sodium butyrate (NaBu) has been used to enhanceprotein expresion levels in mamalian cell culture. Todetermine the clonal variability of recombinant Chinese hamsterovary (rCHO) cels in response to NaBu adition regardingspecific antibody productivity (qAb), three rCHO clones weresubjected to diferent concentrations of NaBu. For all threclones, NaBu addition inhibited cell growth and decreasedthe enhancing effect of NaBu on qAb varied significantly amongthe clones. NaBu addition enhanced the antibo