Jin-Bong Park

Background Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. The amylolytic yeast Saccharomycopsis fibuligera has served as a good source of enzymes and genes involved in saccharification. Despite its long history of use in food fermentation and bioethanol production, very little is known about the basic physiology and genomic features of S. fibuligera. Results We performe

Overall, these data demonstrate that transposon-mediated transgenesis can be applied to cattle without being detrimental to their long-term genomic stability or general health. We further suggest that this technology may be usefully applied in other fields, such as the generation of transgenic animal models.

<i>Lentinula edodes</i> is one of the most popular edible mushrooms in the world and contains useful medicinal components such as lentinan. The whole-genome sequence of <i>L. edodes</i> has been determined with the objective of discovering candidate genes associated with agronomic traits, but experimental verification of gene models with correction of gene prediction errors is lacking. To improve the accuracy of gene prediction, we produced 12.6 Gb of long-read transcriptome data of variable len

The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). The effects of pH, reaction temperature, and non-ionic detergent on recombinant C. glutamiucm whole cell bioconversion were examined. The determined optimal

Gas chromatograph/mass spectrometry (GC/MS)analysis was carried out to determine both the volatile and nonvolatile organic acids in kimchi during 60 days of fermentation at 10oC. Principal component analysis (PCA) was applied to differentiate the pre-defined organic acids and lactic acid bacteria (LAB) during fermentation. Acetic acid was observed as dominant,which was vigorously produced until the middle of fermentation. Lactic acid was the major non-volatile organic acid in the kimchi and was

Considerable progress has been made in functionalization of the soy isoflavones through enzymatic modification of daidzin, genistin, and glycitin. After hydrolysis of β-glucosides into their corresponding aglycones, these compounds were structurally modified via biotransformations such as regioselective hydroxylation, enantioselective reduction, regioselective methylation, and polymerization. These reactions often resulted in an increase of the biological activities (e.g., antioxidative activity

Phospholipase A2 (PLA2) from Streptomyces violaceoruber is a lipolytic enzyme used in a wide range of industrial applications including production of lysolecithins and enzymatic degumming of edible oils. We have therefore investigated expression and secretion of PLA2 in two workhorse microbes, Pichia pastoris and Escherichia coli. The PLA2 was produced to an activity of 0.517 ± 0.012 U/ml in the culture broth of the recombinant P. pastoris. On the other hand, recombinant E. coli BL21 star (DE3),

Acid tolerance is one of the critical factors to determine the quality of the industrial production strains. Therefore, we have investigated the introduction of the acid tolerance genes into the genome of Escherichia coli BL21 by using CRISPR-Cas9 system. The dsrA and rcsB genes of E. coli K-12, which are involved in the heptanoic acid tolerance, were inserted into the genome of E. coli BL21 without scar. The native transcription unit (TU) of dsrA and the synthetic TU of rcsB were integrated in

The effects of cell wall mutation on the oxygenation of linoleic acid (M.W. 280) by recombinant Escherichia coli expressing the CYP102A2 gene encoding self-sufficient P450 monooxygenase of Bacillus subtilis was investigated. After the CYP102A2 gene was heterologously expressed in E. coli W3110 and its isogenic lipopolysaccharide (LPS) structural mutant strains, their whole-cell biotransformation activities were compared. The mutants used in this study had previously been designated as MLK53, MLK

Multiple enzymatic biotransformation of methanol into ethanolamine via formaldehyde and glycolaldehyde was investigated. After discovery of an amine transaminase (ATA), the transamination reaction was connected to condensation of formaldehyde into glycolaldehyde. The ATA from Silicibacter pomeroyi (SpATA), which showed broad substrate spectrum from C4 to C14 aliphatic aldehydes, was able to catalyze the transamination of glycolaldehyde into ethanolamine. The kinetic studies revealed that KM, kca

Solvent stress occurs during whole-cell biocatalysis of organic chemicals. Organic substrates and/or products may accumulate in the cellular membranes of whole cells, causing structural destabilization of the membranes, which leads to disturbances in cellular carbon and energy metabolism. Here, we investigate the effect of cyclohexanone on carbon metabolism in Escherichia coli BL21 and Corynebacterium glutamicum ATCC13032. Adding cyclohexanone to the culture medium (i.e., glucose mineral medium)

TEST 02 - Elsevier's Scopus, the largest abstract and citation database of peer-reviewed literature. Search and access research from the science, technology, medicine, social sciences and arts and humanities fields.

The catalytic activity of oxygenase-based whole-cell biocatalysts is heavily influenced by substrate and product toxicities due to cell membrane permeabilization and protein denaturation effects of the organic substrates and products. Therefore, stability of oxygenase-based whole-cell biocatalysts against solvent stress was investigated with recombinant Escherichia coli BL21 and Corynebacterium glutamicum ATCC13032 expressing the chnB gene of cyclohexanone monooxygenase of Acinetobacter calcoace

To isolate a new P450 monooxygenase belonging to the CYP102 family, CYP102H1 of Nocardia farcinica IFM 10152(i.e., pnf11580) was cloned, expressed, and partially characterized. CYP102H1 gene was amplified from pNF1 of N. farcinica and cloned into expression vectors (i.e., pTrc99A, pET28a(+)). When Escherichia coli BL21(DE3) codon+ strain was transformed with pET28a-CYP102H1 and the culture was induced with 1.0 mM isopropyl-β-D-thio-galactoside in a complex medium at 30oC,CYP102H1 could be expres

A fermenting microorganism involved in formation of ortho-dihydroxyisoflavones (ODIs) during aging ofdoenjang (Korean fermented soybean paste) has been investigated. Microorganisms in ODI-containing doenjang were isolatedby cultivating on yeast mold (YM) agar medium containing 0-7% NaCl. ODI formation of the isolated strains was examinedby gas chromatography/mass spectrometry (GC/MS) analysis after cultivation in modified YM broth or soybean extractmedium. An ODI-producing microbe was identified